BMP-7 Antibody (4E7) recommended for detection of BMP-7 of mouse, rat and human origin by WB, IP and ELISA

BMP-7 Antibody (4E7): sc-53917

BMP-7 Antibody (4E7) is rated 4.4 out of 5 by 7.
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Datasheets

    • Anti-BMP-7 Antibody (4E7) is a mouse monoclonal IgG1 (kappa light chain) BMP-7 antibody provided at 100 µg/ml
    • raised against amino acids 293-431 of BMP-7 of human origin
    • recommended for detection of BMP-7 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BMP-7 (4E7): sc-53917.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with BMP-7 (4E7): sc-53917 antibody?

Asked by: Cweed
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from Did not work for BMMCs IF Tried 1:100 dilution using mouse BMMCs for IF and no signal detected
Date published: 2018-06-21
Rated 5 out of 5 by from Great for WB The BMP-7 Antibody (4E7): sc-53917 works well for Western blot applications, showing clear banding with minimal background.
Date published: 2017-01-26
Rated 5 out of 5 by from Nice signal achieved in WB using MIA PaCa Nice signal achieved in WB using MIA PaCa-2, NTERA-2 cl.D1 and F9 whole cell lysates. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Great WB signal with some background bands Great WB signal with some background bands achieved using F9 whole cell lysate. -SCBT QC
Date published: 2015-02-13
Rated 5 out of 5 by from Very strong band achieved using mouse embryo Very strong band achieved using mouse embryo tissue extract. -SCBT QC
Date published: 2015-01-21
Rated 5 out of 5 by from Good IHC data with paraffin Good IHC data with paraffin-embedded human DAOY cells, DAOY M2.1 cells and MB neoplastic cells. -SCBT Publication Review
Date published: 2015-01-02
Rated 5 out of 5 by from Publishable data in IF with EOC cell lines Publishable data in IF with EOC cell lines. -SCBT Publication Review
Date published: 2014-03-29
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