BMP-6 CRISPR/Cas9 KO Plasmid (h): sc-401979
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMP-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP-6 Antibody (74219.11): sc-57042
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-6 CRISPR/Cas9 KO Plasmid (h) | sc-401979 | 20 µg | $397.00 |
Overview
BMP6 encodes bone morphogenetic protein 6 (BMP-6), a secreted TGF-β superfamily ligand that signals primarily through BMP type I/II serine-threonine kinase receptors to activate SMAD1/5/8-dependent transcriptional programs. BMP-6 contributes to osteogenic and chondrogenic differentiation, extracellular matrix regulation, and tissue remodeling, and it also intersects with developmental patterning and fibrosis-associated signaling contexts. In iron homeostasis, BMP-6 is a key upstream regulator of hepatic hepcidin expression via the BMP/SMAD pathway, linking BMP6 activity to systemic iron balance. Dysregulated BMP6 signaling has been associated with disorders involving aberrant mineralization, fibrosis, and altered iron metabolism, supporting mechanistic studies in relevant cellular models.
BMP-6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BMP6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMP6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMP6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMP-6 protein expression.
This CRISPR knockout system enables efficient generation of BMP6-deficient cell models for investigation of BMP-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMP6 exon(s) critical for BMP-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMP6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMP-6 CRISPR/Cas9 KO Plasmid (h) and BMP-6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMP6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMP-6 HDR Plasmid (h) and BMP-6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMP6 homology arms to support homology-directed repair at defined BMP6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.