Blood Group Wrb Antibody (BRIC14): sc-59476

Blood Group Wrb Antibody (BRIC14) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Blood Group Wrb protein of human origin by immunofluorescence (IF) and flow cytometry (FCM). Blood Group Wrb (BRIC14) monoclonal antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), to suit diverse experimental needs. Blood group antigens, such as the Wrb antigen, play a crucial role in cell recognition and immune response, as they are primarily composed of carbohydrate structures that are added to lipids and proteins on the surface of erythrocytes and certain epithelial cells. The Wrb antigen, specifically, is associated with red blood cell band 3 and glycophorin A, which are essential for maintaining the structural integrity and functionality of red blood cells. Understanding the interactions and modifications of these proteins is vital, as alterations in blood group antigens can influence transfusion compatibility and immune responses in various clinical settings. The Wright (Wr) blood group antigens, including Wra and Wrb, are encoded by alleles of the same gene, and their expression can be modulated during malignant cellular transformation, highlighting their importance in both normal physiology and disease states.

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Blood Group Wrb Antibody (BRIC14) References:

1. Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression.  |  Poole, J., et al. 1999. Transfus Med. 9: 167-74. PMID: 10354388
2. Time-course expression of polypeptides carrying blood group antigens during human erythroid differentiation.  |  Bony, V., et al. 1999. Br J Haematol. 107: 263-74. PMID: 10583211
3. Effect of pronase on high-incidence blood group antigens and the prevalence of antibodies to pronase-treated erythrocytes.  |  Reid, ME., et al. 1996. Immunohematology. 12: 139-42. PMID: 15387724
4. Relationship of the human erythrocyte Wrb antigen to an interaction between glycophorin A and band 3.  |  Telen, MJ. and Chasis, JA. 1990. Blood. 76: 842-8. PMID: 2383660
5. Evidence that Wra and Wrb are antithetical.  |  Wren, MR. and Issitt, PD. 1988. Transfusion. 28: 113-8. PMID: 3281333
6. Effect of pentoxifylline on Wrb antigen.  |  Tweedy, CJ., et al. 1987. Transfusion. 27: 325-9. PMID: 3603661
7. Wright (a + b-) human erythrocytes and Plasmodium falciparum malaria.  |  Hermentin, P., et al. 1985. Blut. 50: 75-80. PMID: 3918597
8. The Wrb antigen in Sta-positive and Dantu-positive human erythrocytes.  |  Ridgwell, K., et al. 1984. J Immunogenet. 11: 365-70. PMID: 6085695
9. Comparative immunochemical analysis of Wra and Wrb red cell antigens.  |  Ring, SM., et al. 1994. Vox Sang. 67: 226-30. PMID: 7801617
10. Changes in the blood group Wright antigens are associated with a mutation at amino acid 658 in human erythrocyte band 3: a site of interaction between band 3 and glycophorin A under certain conditions.  |  Bruce, LJ., et al. 1995. Blood. 85: 541-7. PMID: 7812009
11. Human red blood cell Wright antigens: a genetic and evolutionary perspective on glycophorin A-band 3 interaction.  |  Huang, CH., et al. 1996. Blood. 87: 3942-7. PMID: 8611724
12. Anti-Wrb, and other autoantibodies responsible for positive direct antiglobulin tests in 150 individuals.  |  Issitt, PD., et al. 1976. Br J Haematol. 34: 5-18. PMID: 952768

The preferred WRB antibody is WRB Antibody (G-1): sc-393597.