BLM Antibody (F-5): sc-376616

BLM Antibody (F-5) is a mouse monoclonal IgM antibody that detects BLM in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-BLM antibody (F-5) targets BLM protein, which plays a crucial role in maintaining genomic stability by unwinding DNA during replication and repair processes. This function is particularly important as BLM helps prevent the accumulation of DNA damage that can lead to genomic instability and cancer, a hallmark of Bloom′s syndrome. BLM protein is primarily located in the nucleus, where BLM interacts with both single- and double-stranded DNA, and BLM activity is regulated by ATP binding and hydrolysis. Mutations in the BLM gene can disrupt these essential functions, resulting in an increased frequency of sister chromatid exchanges and a higher susceptibility to various malignancies. BLM (F-5) antibody is an invaluable tool for researchers studying the molecular mechanisms underlying Bloom′s syndrome and related disorders, providing insights into the critical processes of DNA repair and cellular response to genomic stress.

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BLM Antibody (F-5) References:

1. Expression and nuclear localization of BLM, a chromosome stability protein mutated in Bloom's syndrome, suggest a role in recombination during meiotic prophase.  |  Moens, PB., et al. 2000. J Cell Sci. 113 (Pt 4): 663-72. PMID: 10652259
2. Expression of BLM (the causative gene for Bloom syndrome) and screening of Bloom syndrome.  |  Morimoto, W., et al. 2002. Int J Mol Med. 10: 95-9. PMID: 12060858
3. The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis.  |  Stavropoulos, DJ., et al. 2002. Hum Mol Genet. 11: 3135-44. PMID: 12444098
4. Deficiency of Bloom's syndrome protein causes hypersensitivity of C. elegans to ionizing radiation but not to UV radiation, and induces p53-dependent physiological apoptosis.  |  Kim, YM., et al. 2005. Mol Cells. 20: 228-34. PMID: 16267397
5. Solution structure of the HRDC domain of human Bloom syndrome protein BLM.  |  Sato, A., et al. 2010. J Biochem. 148: 517-25. PMID: 20739603
6. Heterozygous germline BLM mutations increase susceptibility to asbestos and mesothelioma.  |  Bononi, A., et al. 2020. Proc Natl Acad Sci U S A. 117: 33466-33473. PMID: 33318203
7. Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.  |  Gönenc, II., et al. 2022. Hum Mol Genet. 31: 2185-2193. PMID: 35099000
8. A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism controlling BLM ubiquitination and G-quadruplex resolution.  |  Liu, S., et al. 2023. Nat Commun. 14: 6111. PMID: 37777511
9. A case of Bloom syndrome manifesting with therapy-related myelodysplastic syndromes harboring a novel BLM gene variant.  |  Ohashi, T., et al. 2024. Int J Hematol. 119: 603-607. PMID: 38489090
10. ESL attenuates BLM-induced IPF in mice: Dual mediation of the TLR4/NF-κB and TGF-β1/PI3K/Akt/FOXO3a pathways.  |  Jia, C., et al. 2024. Phytomedicine. 132: 155545. PMID: 38972238

The preferred BLM antibody is BLM Antibody (B-4): sc-365753.