BLM Antibody (4i317): sc-70426

BLM Antibody (4i317) is a mouse monoclonal IgG1 antibody that detects BLM in human samples through applications such as western blotting (WB) and immunofluorescence (IF). BLM, or Bloom syndrome protein, plays a crucial role in maintaining genomic stability by unwinding DNA during replication and repair processes, which is vital for preventing mutations that can lead to cancer. BLM is primarily located in the nucleus, where BLM interacts with both single- and double-stranded DNA, and BLM′s ATPase activity is essential for its helicase function. Mutations in the BLM gene result in Bloom′s syndrome, characterized by an increased frequency of sister chromatid exchange, which is a hallmark of genomic instability. This instability is linked to a higher predisposition to various cancers, making BLM a significant focus of research in cancer biology and genetics. Anti-BLM antibody (4i317) is an invaluable tool for studying the dynamics of BLM and its implications in disease, providing insights into the mechanisms of DNA repair and the consequences of its dysfunction.

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BLM Antibody (4i317) References:

1. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.  |  Franchitto, A. and Pichierri, P. 2002. J Cell Biol. 157: 19-30. PMID: 11916980
2. Clinical features of Bloom syndrome and function of the causative gene, BLM helicase.  |  Kaneko, H. and Kondo, N. 2004. Expert Rev Mol Diagn. 4: 393-401. PMID: 15137905
3. Transcriptomic and Protein Expression Analysis Reveals Clinicopathological Significance of Bloom Syndrome Helicase (BLM) in Breast Cancer.  |  Arora, A., et al. 2015. Mol Cancer Ther. 14: 1057-65. PMID: 25673821
4. Withdrawal: Selective cleavage of BLM, the Bloom syndrome protein, during apoptotic cell death.  |  Bischof, O., et al. 2020. J Biol Chem. 295: 16905. PMID: 33277404
5. A novel cell-cycle-regulated interaction of the Bloom syndrome helicase BLM with Mcm6 controls replication-linked processes.  |  Shastri, VM., et al. 2021. Nucleic Acids Res. 49: 8699-8713. PMID: 34370039
6. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells.  |  Balbo Pogliano, C., et al. 2022. Sci Adv. 8: eabk0221. PMID: 35119917
7. Design, synthesis and evaluation of N3-substituted quinazolinone derivatives as potential Bloom's Syndrome protein (BLM) helicase inhibitor for sensitization treatment of colorectal cancer.  |  Tu, JL., et al. 2023. Eur J Med Chem. 246: 114944. PMID: 36459756
8. BLM overexpression as a predictive biomarker for CHK1 inhibitor response in PARP inhibitor-resistant BRCA-mutant ovarian cancer.  |  Gupta, N., et al. 2023. Sci Transl Med. 15: eadd7872. PMID: 37343085
9. PARP1 negatively regulates transcription of BLM through its interaction with HSP90AB1 in prostate cancer.  |  Huang, M., et al. 2023. J Transl Med. 21: 445. PMID: 37415147
10. BLM (the causative gene of Bloom syndrome) protein translocation into the nucleus by a nuclear localization signal.  |  Kaneko, H., et al. 1997. Biochem Biophys Res Commun. 240: 348-53. PMID: 9388480

The preferred BLM antibody is BLM Antibody (B-4): sc-365753.