Biotin Antibody (39-15D9): sc-57636

Biotin Antibody (39-15D9) is a mouse monoclonal IgG2b kappa light chain antibody that detects Biotin in non-species reactive applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Biotin antibody (39-15D9) is available in a non-conjugated form, allowing for versatile use in various experimental setups. Biotin, a water-soluble B complex vitamin, plays a crucial role in cellular metabolism, particularly in carboxylation reactions essential for synthesizing fatty acids and glucose, as well as in the metabolism of amino acids like leucine. Biotin′s unique structure, which includes a tetrahydrothiophene ring fused with an ureido ring, is vital for functioning as a coenzyme in carboxylase enzymes, facilitating critical metabolic pathways. While biotin is synthesized by certain microorganisms and plants, human intestinal bacteria also produce biotin, typically meeting daily requirements. Although biotin deficiency is rare, deficiency can lead to significant health issues, including dermatitis, hair loss, and neurological symptoms, underscoring biotin′s importance in maintaining overall health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Biotin Antibody (39-15D9) References:

1. Marginal biotin deficiency is teratogenic.  |  Zempleni, J. and Mock, DM. 2000. Proc Soc Exp Biol Med. 223: 14-21. PMID: 10632957
2. Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.  |  Lu, CS., et al. 2000. J Oral Pathol Med. 29: 445-51. PMID: 11016687
3. The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures.  |  Holmberg, A., et al. 2005. Electrophoresis. 26: 501-10. PMID: 15690449
4. Biotin-ligand complexes with streptavidin quantum dots for in vivo cell labeling of membrane receptors.  |  Lidke, DS., et al. 2007. Methods Mol Biol. 374: 69-79. PMID: 17237530
5. Effect of biotin on activity and gene expression of biotin-dependent carboxylases in the liver of dairy cows.  |  Ferreira, G. and Weiss, WP. 2007. J Dairy Sci. 90: 1460-6. PMID: 17297119
6. High resolution structure of streptavidin in complex with a novel high affinity peptide tag mimicking the biotin binding motif.  |  Perbandt, M., et al. 2007. Proteins. 67: 1147-53. PMID: 17377987
7. Direct effects of radiation on the avidin-biotin system. Absence of energy transfer.  |  Kempner, ES. and Miller, JH. 1990. J Biol Chem. 265: 15776-81. PMID: 2203786
8. Consumer Perception of Biotin Supplementation.  |  John, JJ. and Lipner, SR. 2019. J Cutan Med Surg. 23: 613-616. PMID: 31409115
9. The biotin interference within interference suppressed immunoassays.  |  Kabiri, P., et al. 2021. J Clin Lab Anal. 35: e23940. PMID: 34346119
10. Biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-CoA carboxylase from Brassica napus: cloning and analysis of expression during oilseed rape embryogenesis.  |  Elborough, KM., et al. 1996. Biochem J. 315 (Pt 1): 103-12. PMID: 8670092
11. Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin.  |  Hollinshead, M., et al. 1997. J Histochem Cytochem. 45: 1053-7. PMID: 9267466
12. An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens.  |  Cooper, KM., et al. 1997. Res Vet Sci. 63: 219-25. PMID: 9491447

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Biotin Antibody (39-15D9) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Biotin Antibody (39-15D9) Specifications

Alternate Product Name: CAS 58-85-5

Product Clone Name: 39-15D9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Biotin

Epitope Species:

Product Reactivity/Detection: Biotin

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Biotin Antibody (39-15D9): N/A

The preferred Biotin antibody is Biotin Antibody (BK-1/39): sc-53179.