Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BICD2 CRISPR/Cas9 KO Plasmid (h): sc-405525
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BICD2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BICD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BICD2 Antibody (E-12): sc-393631
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BICD2 CRISPR/Cas9 KO Plasmid (h) | sc-405525 | 20 µg | $397.00 |
Overview
BICD2 (bicaudal D homolog 2) encodes a dynein–dynactin adaptor that couples cargo to microtubule-based minus-end transport, supporting long-range trafficking in polarized cells such as neurons. By linking vesicles, endosomes, and organelles to cytoplasmic dynein, BICD2 contributes to Golgi organization, endosome positioning, nuclear envelope dynamics, and cell cycle-related processes including spindle orientation. BICD2-dependent transport intersects with pathways governing axonal homeostasis and membrane traffic, where precise cargo delivery is required for neuronal maintenance. Genetic variation in BICD2 has been associated with neuromuscular phenotypes, making it a relevant target for studying motor neuron biology and intracellular transport defects.
BICD2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BICD2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BICD2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BICD2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BICD2 protein expression.
This CRISPR knockout system enables efficient generation of BICD2-deficient cell models for investigation of BICD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BICD2 exon(s) critical for BICD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BICD2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BICD2 CRISPR/Cas9 KO Plasmid (h) and BICD2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BICD2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BICD2 HDR Plasmid (h) and BICD2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BICD2 homology arms to support homology-directed repair at defined BICD2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.