BGT-1 Antibody (D-5): sc-514024
- BGT-1 Antibody (D-5) is a mouse monoclonal IgG2b κ BGT-1 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 580-601 within a C-terminal cytoplasmic domain of BGT-1 of mouse origin
- BGT-1 Antibody (D-5) is recommended for detection of BGT-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BGT-1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BGT-1 Antibody (D-5) for WB applications. These reagents are now offered in bundles with BGT-1 Antibody (D-5) (see ordering information below).
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BGT-1 Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects BGT-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BGT-1 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BGT-1, also known as sodium- and chloride-dependent betaine transporter or SLC6A12, consists of 614 amino acids and is characterized as a multi-pass membrane protein. BGT-1 is primarily located in the liver, heart, skeletal muscle, and placenta, with a significant presence in the brain. BGT-1′s structure features twelve transmembrane domains and a large extracellular loop between the third and fourth domains, which is typical of neurotransmitter transporters. This structural configuration enables BGT-1 to transport betaine and gamma-aminobutyric acid (GABA), playing a vital role in regulating GABAergic transmission in the brain through GABA reuptake into presynaptic terminals. BGT-1 is implicated in osmotic regulation and has been associated with mucus production in asthma, potentially influencing the reversibility of lung function abnormalities in patients with aspirin-intolerant asthma. BGT-1 exhibits significant functional differences from other members of the GABA transporter family, highlighting a unique role in neurotransmission and cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BGT-1 Antibody (D-5) References:
- Functional characterization of the Betaine/gamma-aminobutyric acid transporter BGT-1 expressed in Xenopus oocytes. | Matskevitch, I., et al. 1999. J Biol Chem. 274: 16709-16. PMID: 10358010
- Induction of BGT-1 and amino acid system A transport activities in endothelial cells exposed to hyperosmolarity. | Petronini, PG., et al. 2000. Am J Physiol Regul Integr Comp Physiol. 279: R1580-9. PMID: 11049839
- Gamma-aminobutyric acid transporter (BGT-1) expressed in human astrocytoma U373 MG cells: pharmacological and molecular characterization and phorbol ester-induced inhibition. | Ruiz-Tachiquín, ME., et al. 2002. J Neurosci Res. 69: 125-32. PMID: 12111824
- GABA and glutamate transporters are expressed in human platelets. | Rainesalo, S., et al. 2005. Brain Res Mol Brain Res. 141: 161-5. PMID: 16198020
- Association of SLC6A12 variants with aspirin-intolerant asthma in a Korean population. | Pasaje, CF., et al. 2010. Ann Hum Genet. 74: 326-34. PMID: 20597903
- Cloning and expression of a betaine/GABA transporter from human brain. | Borden, LA., et al. 1995. J Neurochem. 64: 977-84. PMID: 7861179
- Re-evaluation of GABA transport in neuronal and glial cell cultures: correlation of pharmacology and mRNA localization. | Borden, LA., et al. 1995. Recept Channels. 3: 129-46. PMID: 8581400
- Osmolyte strategy in human monocytes and macrophages: involvement of p38MAPK in hyperosmotic induction of betaine and myoinositol transporters. | Denkert, C., et al. 1998. Arch Biochem Biophys. 354: 172-80. PMID: 9633613
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BGT-1 Antibody (D-5) | sc-514024 | 200 µg/ml | $322.00 | |||
BGT-1 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-531006 | 200 µg Ab; 10 µg BP | $361.00 | |||
BGT-1 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-524577 | 200 µg Ab, 40 µg BP | $361.00 | |||
BGT-1 Antibody (D-5) AC | sc-514024 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BGT-1 Antibody (D-5) HRP | sc-514024 HRP | 200 µg/ml | $322.00 | |||
BGT-1 Antibody (D-5) FITC | sc-514024 FITC | 200 µg/ml | $336.00 | |||
BGT-1 Antibody (D-5) PE | sc-514024 PE | 200 µg/ml | $349.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 488 | sc-514024 AF488 | 200 µg/ml | $364.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 546 | sc-514024 AF546 | 200 µg/ml | $364.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 594 | sc-514024 AF594 | 200 µg/ml | $364.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 647 | sc-514024 AF647 | 200 µg/ml | $364.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 680 | sc-514024 AF680 | 200 µg/ml | $364.00 | |||
BGT-1 Antibody (D-5) Alexa Fluor® 790 | sc-514024 AF790 | 200 µg/ml | $364.00 | |||
BGT-1 (D-5) Neutralizing Peptide | sc-514024 P | 100 µg/0.5 ml | $69.00 |