Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Bex2 CRISPR/Cas9 KO Plasmid (h): sc-404711
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Bex2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Bex2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Bex2 Antibody (C-12): sc-398486
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bex2 CRISPR/Cas9 KO Plasmid (h) | sc-404711 | 20 µg | $397.00 |
Overview
BEX2 (brain expressed X-linked 2) encodes Bex2, a small, intrinsically disordered protein enriched in neural and other tissues that functions as a context-dependent modulator of transcriptional programs and protein–protein interaction networks. Reported activities place Bex2 at the interface of neurotrophin signaling and stress-response pathways, influencing neuronal differentiation, cell-cycle control, and apoptosis through interactions that can impact MAPK/ERK and related survival signaling. Altered BEX2 expression has been described in several disease-relevant contexts, including neurodevelopmental processes and tumor biology, where it has been associated with changes in proliferation, lineage programs, and treatment response phenotypes. These features make BEX2 a useful node for dissecting signaling-to-transcription coupling and cell-fate decisions in human cellular models.
Bex2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BEX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BEX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BEX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Bex2 protein expression.
This CRISPR knockout system enables efficient generation of BEX2-deficient cell models for investigation of Bex2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BEX2 exon(s) critical for Bex2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BEX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Bex2 CRISPR/Cas9 KO Plasmid (h) and Bex2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BEX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Bex2 HDR Plasmid (h) and Bex2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BEX2 homology arms to support homology-directed repair at defined BEX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.