beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1): sc-81577

β2-AR Antibody (R11E1) is a mouse monoclonal IgG1 kappa light chain antibody that detects β2-adrenergic receptor (β2-AR) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). β2-AR Antibody (R11E1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. β2-AR plays a crucial role in mediating the physiological effects of catecholamines, such as epinephrine and norepinephrine, which are vital for regulating numerous processes including cardiac function, smooth muscle relaxation, and metabolic pathways. Located primarily on the plasma membrane of various cell types, β2-AR is a G protein-coupled receptor that transmits signals through heterotrimeric G proteins, leading to the activation of adenylyl cyclase and subsequent increases in cyclic AMP levels. This signaling pathway is essential for the modulation of heart rate and contractility, as well as the relaxation of bronchial smooth muscle, making β2-AR a significant target for therapeutic interventions in conditions such as asthma and heart failure. The intricate structure of β2-AR, characterized by seven transmembrane helices, allows for specific ligand binding and activation, which is further fine-tuned by post-translational modifications and interactions with various intracellular signaling partners, ensuring precise physiological responses to hormonal stimuli.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) References:

1. From ligand binding to gene expression: new insights into the regulation of G-protein-coupled receptors.  |  Collins, S., et al. 1992. Trends Biochem Sci. 17: 37-9. PMID: 1316650
2. Model systems for the study of seven-transmembrane-segment receptors.  |  Dohlman, HG., et al. 1991. Annu Rev Biochem. 60: 653-88. PMID: 1652922
3. Regulation of adrenergic receptor responsiveness through modulation of receptor gene expression.  |  Collins, S., et al. 1991. Annu Rev Physiol. 53: 497-508. PMID: 2042970
4. The new biology of drug receptors.  |  Lefkowitz, RJ., et al. 1989. Biochem Pharmacol. 38: 2941-8. PMID: 2551299
5. β2-AR signaling controls trastuzumab resistance-dependent pathway.  |  Liu, D., et al. 2016. Oncogene. 35: 47-58. PMID: 25798840
6. Human Brown Adipocyte Thermogenesis Is Driven by β2-AR Stimulation.  |  Blondin, DP., et al. 2020. Cell Metab. 32: 287-300.e7. PMID: 32755608
7. Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway.  |  Guan, Y., et al. 2023. Int J Biol Sci. 19: 2006-2019. PMID: 37151872
8. Macrophage β2-AR activation amplifies inflammation in wound healing by upregulating Trem1 via the cAMP/PKA/CREB pathway.  |  Wei, H., et al. 2024. Int Immunopharmacol. 128: 111463. PMID: 38190789
9. Olodaterol promotes thermogenesis in brown adipocytes via regulation of the β2-AR/cAMP/PKA signaling pathway.  |  Wang, L., et al. 2024. Biochem Biophys Res Commun. 703: 149689. PMID: 38382361
10. An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits.  |  Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
11. Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase.  |  Inglese, J., et al. 1994. Proc Natl Acad Sci U S A. 91: 3637-41. PMID: 8170960
12. Recent perspectives on the molecular structure and regulation of the beta 2-adrenoceptor.  |  Collins, S. 1993. Life Sci. 52: 2083-91. PMID: 8389951

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1) Specifications

Alternate Product Name: ADRB2

Product Clone Name: R11E1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: β₂-AR

Epitope Species: human

Product Reactivity/Detection: β₂-AR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for beta 2 Adrenergic Receptor/ADRB2/β2-AR Antibody (R11E1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin