beta Tubulin Antibody (G-8): sc-55529

β Tubulin Antibody (G-8) is a mouse monoclonal IgG3 kappa light chain antibody designed to target amino acids 210-444 of human β Tubulin, a fundamental component of the cellular cytoskeleton. β Tubulin, also known as β1 Tubulin and β2 Tubulin depending on its isoform, is essential for the formation of microtubules through heterodimerization with α Tubulin. Located primarily in the cytoplasm, β Tubulin plays a critical role in maintaining cell shape, facilitating intracellular transport, and enabling proper cell division, all of which are vital for normal cellular function and organismal development. The precise localization of β Tubulin within the cell underscores its importance in dynamic processes such as mitosis, vesicular trafficking, and cellular motility. anti-β Tubulin antibody (G-8) exhibits broad species reactivity, effectively recognizing β Tubulin in mouse, rat, human, avian, equine, canine, bovine, and porcine samples, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. The existence of multiple β Tubulin isoforms, including the neuron-specific β3 Tubulin, highlights its diverse functional roles in different tissues, and β Tubulin (G-8) antibody facilitates the study of these isoforms in both physiological and pathological contexts. β Tubulin (G-8) monoclonal antibody′s robust performance in multiple assay formats makes this reagent an indispensable tool for researchers investigating cytoskeletal dynamics, cell division mechanisms, and related disease processes.

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beta Tubulin Antibody (G-8) References:

1. Alterations of beta-tubulin isotypes in breast cancer cells resistant to docetaxel.  |  Shalli, K., et al. 2005. FASEB J. 19: 1299-301. PMID: 15946994
2. Physical evidence for cotranslational regulation of beta-tubulin mRNA degradation.  |  Theodorakis, NG. and Cleveland, DW. 1992. Mol Cell Biol. 12: 791-9. PMID: 1732744
3. Expression of beta-tubulin isotypes in human primary ovarian carcinoma.  |  Ohishi, Y., et al. 2007. Gynecol Oncol. 105: 586-92. PMID: 17343904
4. Class III beta-tubulin expression and in vitro resistance to microtubule targeting agents.  |  Stengel, C., et al. 2010. Br J Cancer. 102: 316-24. PMID: 20029418
5. Class III β-tubulin, a novel biomarker in the human melanocyte lineage.  |  Locher, H., et al. 2013. Differentiation. 85: 173-81. PMID: 23817083
6. Dominant effects of tubulin overexpression in Saccharomyces cerevisiae.  |  Burke, D., et al. 1989. Mol Cell Biol. 9: 1049-59. PMID: 2657385
7. Structure-based approaches for the design of benzimidazole-2-carbamate derivatives as tubulin polymerization inhibitors.  |  Aguayo-Ortiz, R., et al. 2017. Chem Biol Drug Des. 90: 40-51. PMID: 28004475
8. Single-Nucleotide Polymorphism Associates' β-Tubulin Isotype-1 Gene in Onchocerca volvulus Populations in Ivermectin-Treated Communities in Taraba State, Nigeria.  |  Akafyi, DE., et al. 2022. Acta Parasitol. 67: 267-274. PMID: 34279775
9. Pyridoxamine and Aminoguanidine Attenuate the Abnormal Aggregation of β-Tubulin and Suppression of Neurite Outgrowth by Glyceraldehyde-Derived Toxic Advanced Glycation End-Products.  |  Ooi, H., et al. 2022. Front Pharmacol. 13: 921611. PMID: 35721214
10. Probing the kinesin-microtubule interaction.  |  Tucker, C. and Goldstein, LS. 1997. J Biol Chem. 272: 9481-8. PMID: 9083088

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta Tubulin Antibody (G-8) has 108 citations in a variety of scientific publications.

Primary antibodies like Anti-beta Tubulin Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta Tubulin Antibody (G-8) Specifications

Alternate Product Name: β Tubulin

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 210-444

Epitope Target: β Tubulin

Epitope Species: human

Product Reactivity/Detection: β Tubulin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 108 citations in various scientific publications

Available Conjugates/Forms for beta Tubulin Antibody (G-8): N/A

The preferred beta Tubulin antibody is beta Tubulin Antibody (D-10): sc-5274.