Anti-beta Tubulin Antibody (D-10) recommended for detection of β Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-β Tubulin Antibody (D-10): sc-5274

Anti-β Tubulin Antibody (D-10) is rated 4.7 out of 5 by 83.
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Datasheets

    • Anti-β Tubulin Antibody (D-10) is a mouse monoclonal IgG2b κ β Tubulin antibody, cited in 464 publications, provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin
    • Anti-beta Tubulin Antibody (D-10) is recommended for detection of β Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta Tubulin Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β Tubulin (D-10): sc-5274.
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Rated 5 out of 5 by from It works very nicely We have used this antibody in the lab for 4 years, and it has always worked nicely. We use a 1:3000 dilution.
Date published: 2021-04-02
Rated 5 out of 5 by from beta-tubulin Ab for WB Excellent antibody for WB dilution 1:50000 good for housekeeping protein of colon cancer cell lines
Date published: 2021-03-23
Rated 2 out of 5 by from discreto funzionamento Questo anticorpo funziona discretamente su cellule fissate con metanolo freddo (-20°C); non funziona in cellule fissate in formalina
Date published: 2021-03-18
Rated 5 out of 5 by from Great b-Tubulin antibody for Western This is a very good mouse monoclonal Ab for beta-Tubulin. We used it with 1:500 dilution and results were very satisfactory and clean. I recommend this antibody.
Date published: 2019-07-30
Rated 2 out of 5 by from Works for HEK293T cells but not for Mouse ES cells I used 1:1000 dilution for cells lysates derived from HEK293T cells. Worked beautifully as an internal loading control. The data sheet says the antibody can recognise mouse, human and rat tubulin. Tried multiple dilutions in mouse ES cells never got a band.
Date published: 2019-07-15
Rated 5 out of 5 by from good control antibody for Western blot We used the antibody for Western blot on whole cell lysate from HEK293T cells. It gives a clean band at the correct size. I recommend the antibody.
Date published: 2019-05-17
Rated 5 out of 5 by from Quality of this antibody This antibody is very good, and works very well in our lab.
Date published: 2019-01-15
Rated 2 out of 5 by from not as expected for a loading control antibody It works. However the quality is not as expected for b.actin that is often used as a loading control. Low quality if compared to other companies
Date published: 2018-12-19
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