β Enolase Antibody (XX-10): sc-100811

β Enolase Antibody (XX-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects beta Enolase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). β Enolase (XX-10) antibody is available as the non-conjugated form. Enolases are highly conserved cytoplasmic glycolytic enzymes that play a crucial role in energy metabolism, particularly in muscle tissue where they facilitate the conversion of phosphoglyceric acid to phosphoenolpyruvic acid in the glycolytic pathway. Among the three isoenzymes—α Enolase, β Enolase, and γ Enolase—β Enolase, also known as Enolase 3 or muscle-specific enolase, is predominantly expressed in adult skeletal muscle, where β Enolase exists as both homodimers and heterodimers with α Enolase. This specific localization and expression pattern are vital for muscle function and energy production, as β Enolase is essential for maintaining the glycolytic flux necessary for muscle contraction and endurance. Mutations in the gene encoding β Enolase can lead to glycogenesis type XIII, a condition characterized by muscle weakness, fatigability, and exercise-induced myalgia, underscoring the importance of β Enolase in muscle health. With detection capabilities across multiple species and applications, anti-beta Enolase antibody (XX-10) serves as an invaluable tool for researchers studying muscle metabolism and related disorders.

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β Enolase Antibody (XX-10) References:

1. Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis.  |  Comi, GP., et al. 2001. Ann Neurol. 50: 202-7. PMID: 11506403
2. Enolase: human tissue distribution and evidence for three different loci.  |  Chen, SH. and Giblett, ER. 1976. Ann Hum Genet. 39: 277-80. PMID: 1275442
3. Beta-enolase in blood plasma during open heart surgery.  |  Usui, A., et al. 1989. Cardiovasc Res. 23: 737-40. PMID: 2514995
4. The need for biochemical testing in beta-enolase deficiency in the genomic era.  |  Wigley, R., et al. 2019. JIMD Rep. 50: 40-43. PMID: 31741825
5. Energy metabolism during exercise in patients with β-enolase deficiency (GSDXIII).  |  Buch, AE., et al. 2021. JIMD Rep. 61: 60-66. PMID: 34485019
6. Serum beta-enolase in acute myocardial infarction.  |  Nomura, M., et al. 1987. Br Heart J. 58: 29-33. PMID: 3620239
7. Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems.  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
8. DNA sequences encoding enolase are remarkably conserved from yeast to mammals.  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
9. Coexpression of alpha and gamma enolase genes in neurons of adult rat brain.  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
10. A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts.  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
11. Expression of two neuronal markers, growth-associated protein 43 and neuron-specific enolase, in rat glial cells.  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
12. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution.  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β Enolase Antibody (XX-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-β Enolase Antibody (XX-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β Enolase Antibody (XX-10) Specifications

Alternate Product Name: ENO3

Product Clone Name: XX-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 228-278

Epitope Target: β Enolase

Epitope Species: human

Product Reactivity/Detection: β Enolase (muscle)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for β Enolase Antibody (XX-10): N/A

The preferred Enolase antibody is Enolase Antibody (A-5): sc-271384.