beta-catenin Antibody (E-2): sc-515074

β-catenin Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect β-catenin, also known as CTNNB1, a critical protein involved in both cellular adhesion and gene transcription regulation. β-catenin is a key component of the cadherin protein complex at the cell membrane, where β-catenin interacts with E-cadherin to maintain cell-cell adhesion and tissue integrity. Beyond its structural role, β-catenin is central to the Wnt signaling pathway, where β-catenin undergoes essential post-translational modifications such as phosphorylation and ubiquitination. Phosphorylation of β-catenin by kinases like GSK-3β targets β-catenin for ubiquitination and subsequent degradation, thereby regulating β-catenin levels and activity within the cell. These modifications are crucial for controlling cell proliferation, differentiation, and migration, processes that are fundamental to normal development and are often disrupted in cancerous transformations. Anti-β-catenin antibody (E-2) specifically recognizes an epitope between amino acids 684-707 near the C-terminus of human β-catenin, ensuring precise and reliable detection in multiple species including mouse, rat, and human. β-catenin monoclonal antibody (E-2) is validated for various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), making β-catenin (E-2) antibody a versatile tool for diverse research needs. By enabling the detailed study of β-catenin′s post-translational modifications and their impact on cellular functions, β-catenin (E-2) monoclonal antibody is an indispensable reagent for researchers investigating the molecular mechanisms underlying cellular communication, development, and oncogenesis.

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.