beta-catenin Antibody (12F7): sc-59737

β-catenin Antibody (12F7) is a mouse monoclonal IgG1 kappa light chain antibody developed against recombinant human β-catenin, effectively detecting β-catenin from mouse, rat, and human origins in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-β-catenin antibody (12F7) offers versatility for diverse experimental needs. β-catenin, also known as catenin beta-1 or CTNNB1, is a multifunctional protein integral to cell-cell adhesion and gene transcription regulation. Post-translational modifications (PTMs) of β-catenin, such as phosphorylation, ubiquitination, and acetylation, are crucial for function and regulation. Phosphorylation at specific serine and threonine residues by kinases like GSK-3β and CK1 leads to β-catenin′s recognition by the β-TrCP ubiquitin ligase, marking β-catenin for proteasomal degradation and thus regulating cytoplasmic levels. Conversely, dephosphorylation or mutations at these sites can result in β-catenin stabilization, accumulation, and translocation to the nucleus, where β-catenin interacts with TCF/LEF transcription factors to modulate gene expression involved in cell proliferation and differentiation. Dysregulation of β-catenin′s PTMs is implicated in various cancers, highlighting the importance of these modifications in maintaining cellular homeostasis. β-catenin (12F7) antibody is an indispensable tool for researchers investigating these critical cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta-catenin Antibody (12F7) has 120 citations in a variety of scientific publications.

Primary antibodies like Anti-beta-catenin Antibody (12F7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta-catenin Antibody (12F7) Specifications

Alternate Product Name: β-catenin

Product Clone Name: 12F7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: β-catenin

Epitope Species: human

Product Reactivity/Detection: β-catenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 120 citations in various scientific publications

Available Conjugates/Forms for beta-catenin Antibody (12F7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.