β-Arrestin-2 Antibody (H-9): sc-13140

β-Arrestin-2 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to detect β-Arrestin-2, also known as ARRB2, across a variety of species including human, mouse, rat, canine, bovine, and porcine. Anti-β-Arrestin-2 antibody (H-9) recognizes an epitope located between amino acids 2-33 at the N-terminus of the human β-Arrestin-2 protein, ensuring reliable detection in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). β-Arrestin-2 is a critical regulator of G protein-coupled receptor (GPCR) signaling pathways, mediating receptor desensitization, internalization, and initiating alternative signaling cascades independent of G proteins. This multifunctional protein is predominantly expressed in neuronal and spleen tissues, where β-Arrestin-2 plays a vital role in modulating neurotransmitter receptor activity and cellular responses to various signaling molecules. β-Arrestin-2′s ability to influence diverse physiological processes makes β-Arrestin-2 a key target for research in areas such as neurotransmission, inflammation, and cancer progression. Understanding the interactions and regulatory mechanisms of β-Arrestin-2 is essential for developing therapeutic strategies aimed at these critical pathways. β-Arrestin-2 (H-9) antibody is available in both conjugated and unconjugated forms, offering versatility to meet different experimental needs.

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β-Arrestin-2 Antibody (H-9) References:

1. Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family.  |  Attramadal, H., et al. 1992. J Biol Chem. 267: 17882-90. PMID: 1517224
2. Two kinases mediate agonist-dependent phosphorylation and desensitization of the beta 2-adrenergic receptor.  |  Hausdorff, WP., et al. 1990. Symp Soc Exp Biol. 44: 225-40. PMID: 1983369
3. Multiple second messenger pathways of alpha-adrenergic receptor subtypes expressed in eukaryotic cells.  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
4. β-Arrestin-2 BRET Biosensors Detect Different β-Arrestin-2 Conformations in Interaction with GPCRs.  |  Oishi, A., et al. 2020. ACS Sens. 5: 57-64. PMID: 31849219
5. β-arrestin 2 as an activator of cGAS-STING signaling and target of viral immune evasion.  |  Zhang, Y., et al. 2020. Nat Commun. 11: 6000. PMID: 33243993
6. β-Arrestin 2 Regulates Inflammatory Responses against Mycobacterium tuberculosis Infection through ERK1/2 Signaling.  |  Wen, Q., et al. 2021. J Immunol. 206: 2623-2637. PMID: 34001657
7. β-arrestin-1 and β-arrestin-2 Restrain MRGPRX2-Triggered Degranulation and ERK1/2 Activation in Human Skin Mast Cells.  |  Wang, Z., et al. 2022. Front Allergy. 3: 930233. PMID: 35910860
8. NE-activated β2-AR/β-arrestin 2/Src pathway mediates duodenal hyperpermeability induced by water-immersion restraint stress.  |  Yan, JT., et al. 2023. Am J Physiol Cell Physiol. 324: C133-C141. PMID: 36440855
9. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function.  |  Zaïmia, N., et al. 2023. Cell Rep. 42: 113326. PMID: 37897727
10. The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
11. Arrestin function in inactivation of G protein-coupled receptor rhodopsin in vivo.  |  Dolph, PJ., et al. 1993. Science. 260: 1910-6. PMID: 8316831
12. Molecular analysis of human beta-arrestin-1: cloning, tissue distribution, and regulation of expression. Identification of two isoforms generated by alternative splicing.  |  Parruti, G., et al. 1993. J Biol Chem. 268: 9753-61. PMID: 8486659

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-Arrestin-2 Antibody (H-9) has 99 citations in a variety of scientific publications.

Primary antibodies like Anti-β-Arrestin-2 Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-Arrestin-2 Antibody (H-9) Specifications

Alternate Product Name: beta Arrestin

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-33

Epitope Target: β-Arrestin-2

Epitope Species: human

Product Reactivity/Detection: β-Arrestin-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 99 citations in various scientific publications

Available Conjugates/Forms for β-Arrestin-2 Antibody (H-9): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, P: Blocking Peptide, PE: Phycoerythrin