beta Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is a mouse monoclonal IgG1 kappa light chain antibody specifically raised against chicken gizzard actin. Anti-β-actin antibody (C4) is recommended for detecting β-actin in various species, including mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum, and Physarum polycephalum, by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). β-Actin is a critical component of the cytoskeleton in eukaryotic cells, contributing to cell structure, motility, and division. β-Actin′s high conservation across species makes anti-β-actin antibody (C4) ideal for cross-reactivity studies in both mammalian and non-mammalian cells. Actin filaments form the dynamic backbone of cellular structures like microvilli and are essential for muscle cell contractility. β-Actin and γ-actin, the primary cytoplasmic isoforms, are found in a wide range of tissues, while α-actin is primarily expressed in muscle cells. Small GTPase family members, including Rho, Rac, and Cdc42, regulate actin filament organization, controlling cellular processes like stress fiber formation, membrane ruffling, and filopodia formation. Post-translational modifications of β-actin include arginylation, where arginyltransferase (ATE1) adds an arginine residue to β-actin. This arginylation plays a crucial role in regulating actin cytoskeleton dynamics, essential for cell movement, shape, and adhesion. β-Actin arginylation influences interactions with other cytoskeletal proteins, affecting cell migration and tissue development. Dysregulation of β-actin arginylation can contribute to diseases like cancer, where altered cell motility and invasion are key factors. β-Actin monoclonal antibody (C4) is available in non-conjugated forms and conjugated versions with agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, offering versatility for various laboratory applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

beta Actin Antibody (C4) References:

1. Imaging native beta-actin mRNA in motile fibroblasts.  |  Tyagi, S. and Alsmadi, O. 2004. Biophys J. 87: 4153-62. PMID: 15377515
2. Two monoclonal antibodies to actin: one muscle selective and one generally reactive.  |  Lessard, JL. 1988. Cell Motil Cytoskeleton. 10: 349-62. PMID: 2460261
3. Expression of transfected mutant beta-actin genes: transitions toward the stable tumorigenic state.  |  Leavitt, J., et al. 1987. Mol Cell Biol. 7: 2467-76. PMID: 3614199
4. Role of microtubule-associated proteins in the control of microtubule assembly.  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
5. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
6. A discourse on modeling F-actin.  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
7. Actin cytoskeleton. Setting the pace of cell movement.  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
8. The origins and evolution of eukaryotic proteins.  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
9. Elastic fibres are an essential component of human placental stem villous stroma and an integrated part of the perivascular contractile sheath.  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
10. Actin bundles in human hair follicles as revealed by confocal laser microscopy.  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672
11. The structure of an open state of beta-actin at 2.65 A resolution.  |  Chik, JK., et al. 1996. J Mol Biol. 263: 607-23. PMID: 8918942

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta Actin Antibody (C4) has 15,023 citations in a variety of scientific publications.

Primary antibodies like Anti-beta Actin Antibody (C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta Actin Antibody (C4) Specifications

Alternate Product Name: β-Actin

Product Clone Name: C4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: gizzard Actin

Epitope Species: chicken

Product Reactivity/Detection: β-Actin

Species Reactivity/Detection: mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum, Physarum polycephalum

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15,023 citations in various scientific publications

Available Conjugates/Forms for beta Actin Antibody (C4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent