Anti-beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-Actin Antibody (C4): sc-47778

Anti-β-Actin Antibody (C4) is rated 4.8 out of 5 by 434.
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Datasheets

    • Anti-β-Actin Antibody (C4) is a mouse monoclonal IgG1 κ β-Actin antibody, cited in 12,570 publications, provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • Anti-beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • Anti-beta-Actin Antibody (C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-Actin Antibody (C4) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Actin Antibody (C4) (see ordering information below).
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你好,近期使用这只抗体发现条带位置不对,在三十五到四十之间,并且有双条带。40-55kd的位置没有条带,请问可能是什么原因

Asked by: 卡卡何不短了
感谢您的提问。您可以拨打电话:021-60936350,发送邮件到[email protected],或者我们官网上的在线聊天,联系我们的亚洲技术支持团队,了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-08-17

Is Anti-β-Actin Antibody (C4): sc-47778 can be used to detect Beta-actin protein using Secondary antibody m-IgGκ BP-HRP: sc-516102 in Rat, Mice tissues and Human cell protein samples

Asked by: N Kumar
Thank you for your question. Yes, those products are compatible for your intended purpose.
Answered by: Tech Support Europe
Date published: 2022-08-03

Hello, I’d like to test if this antibody works on several species of foraminifera (Quinqueloculina sp., Ammonia sp., Amphistegina lessonii). How can I apply for the trial sample?

Asked by: Jan P Golen
Thank you for your question. Please contact our Technical Service Department (or your local Distributor) directly to arrange for a free 10 µg sample of β-Actin (C4): sc-47778.
Answered by: Tech Support Europe
Date published: 2022-01-19

I will use this Ab as loading control in WB by H157 cells. can this be used in H157 cells ?

Asked by: bocchi
Thank you for your inquiry. Anti-beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform). Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2021-10-09

What are the amino acids that the antibody recognizes?

Asked by: Anonymous
Thank you for your question. This antibody was raised against the full length gizzard Actin of chicken origin, accession# P60706. We have not characterized the exact binding site of the antibody.
Answered by: Blake J
Date published: 2021-07-12

For sc-47778, do you sell a matched negative control mouse IgG1 or blocking peptide for Western blotting?

Asked by: Ma1234
Thank you for your question. We do not offer a blocking peptide for this antibody, but normal mouse IgG1: sc-3877 can be used as an isotype control.
Answered by: Technical Support Europe
Date published: 2020-10-08

Hello, I am using Beta Actin antibody for Peromyscus (deer mice) but I always have lots of unspecific bands. I was wondering has any boy else used it before for deer mice and did they have the same experiment?

Asked by: Eliie
Thank you for your question. Unfortunately we are not aware of any testing on deer mice using this antibody. Please contact technical service directly for assistance regarding your WB results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-12-09

Can anyone recommend an santa cruz anti-actin antibody for detection with plant material?

Asked by: MichaelN
Thank you for your question. This antibody clone has previously been published in arabidopsis samples, so it would be a good choice.
Answered by: Tech Service
Date published: 2019-08-14
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Rated 4 out of 5 by from Actin antibody Antibody is good. Use in dilution less then 1;1000 to get better and discrete signal.
Date published: 2022-07-12
Rated 5 out of 5 by from Good antibody Good quality. I have used it for WB and it's working great!
Date published: 2022-07-08
Rated 5 out of 5 by from Great antibody We have been using this antibody for long time and its very good.
Date published: 2022-06-27
Rated 5 out of 5 by from β-Actin antibody This antibody is very good, our lab will continue to buy it!
Date published: 2022-06-20
Rated 5 out of 5 by from Great actin antibody We use beta-actin as a loading control on our Western blots and have been very happy with this antibody. It reliably gives a strong signal. We use it at 1:500 dilution for ~3 hr at RT. Highly recommend this one.
Date published: 2022-06-10
Rated 5 out of 5 by from good for WB bone tissue WB dilution 1:1000 used for more than 4 times (overnight incubation) and still good signals
Date published: 2022-02-12
Rated 5 out of 5 by from Great features We applied this antibody to do western blot and found it is suitable for WB
Date published: 2022-01-11
Rated 5 out of 5 by from Excellent detection in WB The band is strong and could re-use the antibody at 1.1000 dilution for at least three times!
Date published: 2022-01-06
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