Anti-beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-Actin Antibody (C4): sc-47778

Anti-β-Actin Antibody (C4) is rated 4.8 out of 5 by 418.
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Datasheets

    • Anti-β-Actin Antibody (C4) is a mouse monoclonal IgG1 κ β-Actin antibody, cited in 10,926 publications, provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • Anti-beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • Anti-beta-Actin Antibody (C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-Actin Antibody (C4) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Actin Antibody (C4) (see ordering information below).
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What are the amino acids that the antibody recognizes?

Asked by: Anonymous
Thank you for your question. This antibody was raised against the full length gizzard Actin of chicken origin, accession# P60706. We have not characterized the exact binding site of the antibody.
Answered by: Blake J
Date published: 2021-07-12

For sc-47778, do you sell a matched negative control mouse IgG1 or blocking peptide for Western blotting?

Asked by: Ma1234
Thank you for your question. We do not offer a blocking peptide for this antibody, but normal mouse IgG1: sc-3877 can be used as an isotype control.
Answered by: Technical Support Europe
Date published: 2020-10-08

Hello, I am using Beta Actin antibody for Peromyscus (deer mice) but I always have lots of unspecific bands. I was wondering has any boy else used it before for deer mice and did they have the same experiment?

Asked by: Eliie
Thank you for your question. Unfortunately we are not aware of any testing on deer mice using this antibody. Please contact technical service directly for assistance regarding your WB results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-12-09

Can anyone recommend an santa cruz anti-actin antibody for detection with plant material?

Asked by: MichaelN
Thank you for your question. This antibody clone has previously been published in arabidopsis samples, so it would be a good choice.
Answered by: Tech Service
Date published: 2019-08-14

What blocking buffer can be used for this antibody? Is 1X TBST with 5% BSA okay to detect bands?

Asked by: Edd22
Thank you for your question. We recommend 5% milk with TBS-T for most Westen blot applications, alternatively we also suggest using our UltraCruz Blocking reagent. You can find them both here: https://www.scbt.com/scbt/browse/Blocking-Reagents-for-Western-Blotting-Applications/_/N-1hrinjk
Answered by: Tech Service
Date published: 2019-11-18

Would this antibody recognize actin in filament (F-actin) or subunit (G-actin)?

Asked by: Anonymous
Thank you for your question. This antibody recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin.
Answered by: Technical Support
Date published: 2020-07-03

Would this antibody also recognize G-actin?

Asked by: XC268
Thank you for your question. If by G-actin you are referring to gamma-actin (γ- Actin) then this antibody may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform).
Answered by: Technical Support
Date published: 2019-03-03

β-actin (C4)抗体可以用于昆虫细胞吗,尤其蚊虫细胞

Asked by: 蛋白果果虫
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-07-03
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Rated 4 out of 5 by from Worked very well A very clear signal was obtained in western blot. Was used on an 10% acrylamide gel
Date published: 2021-09-20
Rated 5 out of 5 by from Great antibody! Great antibody and I got great data from this! Will use this in future work!
Date published: 2021-09-16
Rated 5 out of 5 by from Good for use as western blot loading control Good signal in rat placental tissue when used at a 1:1000 dilution. Secondary antibody was sc-2005.
Date published: 2021-08-12
Rated 5 out of 5 by from Fabulous features! Bands seen in WB using human tracheal smooth muscle whole cell lysates.
Date published: 2021-07-21
Rated 5 out of 5 by from Clean bands Tested in NCI-H660 cells western blot and bands were clear and clean
Date published: 2021-07-09
Rated 5 out of 5 by from Great antibody p70s6 : sc-8418 andp-p70s6: sc-8416 I tried samples and then bought full size pack of the above antibodies and they are super sensitive for Western blot and ICC. I strongly recommend this antibodies for these aplications.
Date published: 2021-05-19
Rated 5 out of 5 by from It is suitable for western blot It is suitable for western blot, therefore, I suggest this antibody to perform western blot
Date published: 2021-05-11
Rated 5 out of 5 by from Reliably detects actin in Dictyostelium Reliably detects actin in Dictyostelium whole cell lysates
Date published: 2021-04-09
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