beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 397.
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Datasheets

    • Anti-β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) β-Actin antibody provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • Anti-beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • Anti-beta-Actin Antibody (C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.

Hello, I am using Beta Actin antibody for Peromyscus (deer mice) but I always have lots of unspecific bands. I was wondering has any boy else used it before for deer mice and did they have the same experiment?

Asked by: Eliie
Thank you for your question. Unfortunately we are not aware of any testing on deer mice using this antibody. Please contact technical service directly for assistance regarding your WB results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-12-09

Can anyone recommend an santa cruz anti-actin antibody for detection with plant material?

Asked by: MichaelN
Thank you for your question. This antibody clone has previously been published in arabidopsis samples, so it would be a good choice.
Answered by: Tech Service
Date published: 2019-08-14

What blocking buffer can be used for this antibody? Is 1X TBST with 5% BSA okay to detect bands?

Asked by: Edd22
Thank you for your question. We recommend 5% milk with TBS-T for most Westen blot applications, alternatively we also suggest using our UltraCruz Blocking reagent. You can find them both here: https://www.scbt.com/scbt/browse/Blocking-Reagents-for-Western-Blotting-Applications/_/N-1hrinjk
Answered by: Tech Service
Date published: 2019-11-18

Would this antibody recognize actin in filament (F-actin) or subunit (G-actin)?

Asked by: Anonymous
Thank you for your question. This antibody recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin.
Answered by: Technical Support
Date published: 2019-03-21

Would this antibody also recognize G-actin?

Asked by: XC268
Thank you for your question. If by G-actin you are referring to gamma-actin (γ- Actin) then this antibody may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform).
Answered by: Technical Support
Date published: 2019-03-03

β-actin (C4)抗体可以用于昆虫细胞吗,尤其蚊虫细胞

Asked by: 蛋白果果虫
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-07-03

We would like to get a 10ug free antibody sample for beta actin sc-47778. we are planning to use that antibody for westernblooting. the templete protein is RBC membrane We used to use Actin(C-11): sc-1615, and want to get antibody with similar working.

Asked by: Sakurako Neo
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-02-08

Is this antibody working in NON REDUCING conditions in Western Blot?

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
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Rated 5 out of 5 by from B-actin antibody applied to meagre aquaculture B-actin protein was detected in several tissues of meagre (Argyrosomus regius) by using the b-actin antibody. B-actin was detected in the intestine, liver and white muscle fish samples. This antibody provided a novel tool to semi-quantify proteins in meagre by western blot.
Date published: 2020-07-03
Rated 5 out of 5 by from Great product! This antibody works well without any nonspecific bands and background.
Date published: 2020-03-19
Rated 5 out of 5 by from clear signal in primary human fibroblasts The antibody gives a strong and clear bands in primary human skin fibroblasts. Dilution 1:1000, incubation 1h in 5% milk in TBST
Date published: 2020-02-25
Rated 5 out of 5 by from Excellent for WB Works excellent for WB in Human Trophoblast derived cells. Single band, strong signal, clean.
Date published: 2020-02-18
Rated 5 out of 5 by from Good Antibody in WB We used this antibody in WB analysis and it works very well at 1:1000 dilution for both murine and human cell extracts
Date published: 2020-02-07
Rated 5 out of 5 by from great antibody Single band, no other bands with BioVision ECL on western blot
Date published: 2020-02-06
Rated 5 out of 5 by from 条带清晰 我们使用这个抗体作为内参很满意,条带清晰,稳定可重复。我是按照1:1000配置的,采用PBS即可。可以重读多次使用。
Date published: 2020-01-06
Rated 5 out of 5 by from Great antibody for loading control I use this antibody at 1:1000 dilution for loading control in Western blotting. It gives a very specific and clean band at 45 KDa, recomend!
Date published: 2019-12-06
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