beta Actin Antibody (4E8H3): sc-130065

β-Actin Antibody (4E8H3) is a mouse monoclonal IgG1 kappa light chain antibody, raised against a peptide mapping at the N-terminus of human β-Actin, and is reactive with mouse, rat, and human species. Anti-β-Actin antibody (4E8H3) is ideal for applications such as western blotting (WB) and enzyme-linked immunosorbent assays (ELISA). β-Actin is a highly conserved protein that constitutes up to 50% of total cellular protein in eukaryotic cells and plays a critical role in maintaining cell structure and motility. Located predominantly in the cytoplasm, β-Actin is a principal component of the cytoskeleton, forming microfilaments that are essential for maintaining cell shape, enabling cell movement, and facilitating intracellular transport. β-Actin exists in a dynamic equilibrium between monomeric globular actin (G-actin) and filamentous actin (F-actin), allowing for rapid reorganization of the cytoskeleton in response to cellular signals. This reorganization is crucial for processes such as muscle contraction, cell division, and cell signaling. The regulation of β-Actin polymerization and depolymerization is controlled by small GTPase family members like Rho, Rac, and Cdc42, which influence the formation of actin stress fibers, membrane ruffles, and filopodia, respectively. These processes are vital for cell adhesion, migration, and morphology. Given its ubiquitous expression and essential functions, β-Actin serves as a reliable loading control in various assays, making β-Actin (4E8H3) antibody a valuable tool for researchers studying protein expression and cellular dynamics.

The preferred beta Actin antibody is beta Actin Antibody (C4): sc-47778.