β-1,4-Gal-T1 Antibody (A-3): sc-515551

β-1,4-Gal-T1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects β-1,4-Gal-T1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-β-1,4-Gal-T1 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The β-1,4-Gal-T1 gene, located on chromosome 9p13, is one of seven genes encoding beta-1,4-galactosyltransferases, which are type II membrane-bound glycoproteins that specifically utilize UDP-galactose as a donor substrate. These proteins are crucial for the transfer of galactose in a beta-1,4 linkage to acceptor sugars such as N-acetylglucosamine, glucose, and xylose, playing a significant role in the biosynthesis of glycoconjugates. β-1,4-Gal-T1 is unique among its family as β-1,4-Gal-T1 is involved in both glycoconjugation and lactose biosynthesis, highlighting its importance in cellular processes. Two transcripts of approximately 4.1 kb and 3.9 kb encode β-1,4-Gal-T1, differing only at their 5′ ends; the longer transcript encodes the membrane-bound form that resides in the trans-Golgi network, while the shorter transcript produces a soluble form that contributes to lactose synthase activity. This dual functionality underscores β-1,4-Gal-T1′s critical role in both glycan structure formation and lactation, making β-1,4-Gal-T1 a vital component in various biological processes.

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β-1,4-Gal-T1 Antibody (A-3) References:

1. Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.  |  Amado, M., et al. 1999. Biochim Biophys Acta. 1473: 35-53. PMID: 10580128
2. Involvement of beta 1,4 galactosyltransferase 1 and Gal beta1-->4GlcNAc groups in human hepatocarcinoma cell apoptosis.  |  Zhang, S., et al. 2003. Mol Cell Biochem. 243: 81-6. PMID: 12619892
3. Cell surface beta 1, 4-galactosyltransferase 1 promotes apoptosis by inhibiting epidermal growth factor receptor pathway.  |  Li, Z., et al. 2006. Mol Cell Biochem. 291: 69-76. PMID: 16786197
4. Association of beta-1,3-N-acetylglucosaminyltransferase 1 and beta-1,4-galactosyltransferase 1, trans-Golgi enzymes involved in coupled poly-N-acetyllactosamine synthesis.  |  Lee, PL., et al. 2009. Glycobiology. 19: 655-64. PMID: 19261593
5. β1,4-galactosyltransferase 1 is a novel receptor for IgA in human mesangial cells.  |  Molyneux, K., et al. 2017. Kidney Int. 92: 1458-1468. PMID: 28750925
6. Golgi and secreted galactosyltransferase.  |  Strous, GJ. 1986. CRC Crit Rev Biochem. 21: 119-51. PMID: 3093147
7. The receptor function of galactosyltransferase during mammalian fertilization.  |  Shur, BD. 1986. Adv Exp Med Biol. 207: 79-93. PMID: 3103381
8. Analysis of homologous and heterologous interactions between UDP-galactose transporter and beta-1,4-galactosyltransferase 1 using NanoBiT.  |  Wiertelak, W., et al. 2020. Anal Biochem. 593: 113599. PMID: 32004544
9. β-1,4-Galactosyltransferase 1 protects against cerebral ischemia injury in mice by suppressing ferroptosis via the TAZ/Nrf2/HO-1 signaling pathway.  |  Ma, Y., et al. 2024. CNS Neurosci Ther. 30: e70030. PMID: 39233353
10. Molecular Mechanisms Underlying the Loop-Closing Dynamics of β-1,4 Galactosyltransferase 1.  |  Tian, J., et al. 2025. J Chem Inf Model. 65: 390-401. PMID: 39737871
11. The receptor function of galactosyltransferase during cellular interactions.  |  Shur, BD. 1984. Mol Cell Biochem. 61: 143-58. PMID: 6427602
12. A family of human beta3-galactosyltransferases. Characterization of four members of a UDP-galactose:beta-N-acetyl-glucosamine/beta-nacetyl-galactosamine beta-1,3-galactosyltransferase family.  |  Amado, M., et al. 1998. J Biol Chem. 273: 12770-8. PMID: 9582303

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-1,4-Gal-T1 Antibody (A-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-β-1,4-Gal-T1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-1,4-Gal-T1 Antibody (A-3) Specifications

Alternate Product Name: β-1,4-Gal-T1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-160

Epitope Target: β-1,4-Gal-T1

Epitope Species: human

Product Reactivity/Detection: β-1,4-Gal-T1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for β-1,4-Gal-T1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin