Bestrophin Antibody (E6-6) recommended for detection of Bestrophin of human origin by WB, IP, IF and IHC(P)

Bestrophin Antibody (E6-6): sc-32792

Bestrophin Antibody (E6-6) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-Bestrophin Antibody (E6-6) is a mouse monoclonal IgG1 (kappa light chain) Bestrophin antibody provided at 200 µg/ml
    • raised against a synthetic peptide corresponding to the C-terminus of human bestrophin
    • Bestrophin Antibody (E6-6) is recommended for detection of Bestrophin of human origin by WB, IP, IF and IHC(P); non cross-reactive with mouse or rat Bestrophin
    • Anti-Bestrophin Antibody (E6-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bestrophin (E6-6): sc-32792.

Hi, My question is: Do you know what is the epitope sequence from sc-32792? ... Can you tell me?, because I can not see in information page about this antibody. Thanks

Asked by: Alejandro789
Thank you for your question. According to the literature, this monoclonal antibody, clone E6-6, was raised against peptide immunogen: KDHMDPYWALENRDEAHS. PMID: 11050159.
Answered by: Technical Support
Date published: 2019-08-16

What is the recommended starting dilution for Western blot for sc-32792: Bestrophin (E6-6) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 2 out of 5 by from Antibodies are not working Dear, I am sorry to say that this antibody did not work and gave me a lot of background. Of course using mice antibodies on mice tissue is not at all the best, but in my hand I had other antibodies working with less background. And moreover, the expected signal was not at all present, while the positive control worked very well. I tried 2 different dilutions 1:100 and 1: 500, but same results. I am sorry to say that, because usually Santa Cruz antibodies work very well...
Date published: 2018-03-29
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