BECN1/Beclin-1 Antibody (E-8): sc-48341

Beclin 1 Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody engineered to recognize Beclin 1 in mouse, rat, and human species, making anti-Beclin 1 antibody (E-8) an essential tool for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Beclin 1 monoclonal antibody (E-8) is raised against amino acids 1-300 of human Beclin 1 and is available in both non-conjugated and various conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental needs. Beclin 1, also called BECN1, ATG6, VPS30, and beclin1, is a pivotal coiled-coil protein that plays a critical role in autophagy regulation, a fundamental cellular process responsible for degrading and recycling cellular components within lysosomes. By interacting with Bcl-2, Beclin 1 modulates autophagic activity, which is essential for maintaining cellular homeostasis and preventing uncontrolled cell growth, thereby acting as a key inhibitor of tumorigenesis. Reduced Beclin 1 expression in various carcinoma cell lines, such as MCF7, compared to ubiquitous expression in normal tissues, highlights a role in suppressing cancer development. Additionally, Beclin 1 shares structural similarities with yeast autophagy gene product Apg6, emphasizing conserved function in autophagy across different organisms. Beclin 1′s ability to complement autophagocytosis and inhibit cellular proliferation in transfected cells further illustrates significance in regulating cell growth and maintaining cellular integrity. Beclin 1 (E-8) antibody serves as an indispensable reagent for researchers studying molecular mechanisms of autophagy, cancer biology, and related cellular processes, providing reliable and versatile detection in various experimental settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BECN1/Beclin-1 Antibody (E-8) References:

1. Induction of autophagy and inhibition of tumorigenesis by beclin 1.  |  Liang, XH., et al. 1999. Nature. 402: 672-6. PMID: 10604474
2. The Beclin 1 network regulates autophagy and apoptosis.  |  Kang, R., et al. 2011. Cell Death Differ. 18: 571-80. PMID: 21311563
3. Beclin 1 biology and its role in heart disease.  |  Zhu, H. and He, L. 2015. Curr Cardiol Rev. 11: 229-37. PMID: 25373623
4. Disruption of the beclin 1-BCL2 autophagy regulatory complex promotes longevity in mice.  |  Fernández, ÁF., et al. 2018. Nature. 558: 136-140. PMID: 29849149
5. Beclin-1-Dependent Autophagy Protects the Heart During Sepsis.  |  Sun, Y., et al. 2018. Circulation. 138: 2247-2262. PMID: 29853517
6. Aldehyde dehydrogenase 2 protects against acute kidney injury by regulating autophagy via the Beclin-1 pathway.  |  Xu, T., et al. 2021. JCI Insight. 6: PMID: 34228649
7. USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity.  |  Li, J., et al. 2022. Nat Commun. 13: 7799. PMID: 36528652
8. Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging.  |  Guo, R., et al. 2024. Nat Struct Mol Biol. 31: 232-245. PMID: 38177673
9. Phosphorylation of ribosomal protein S6 is inhibitory for autophagy in isolated rat hepatocytes.  |  Blommaart, EF., et al. 1995. J Biol Chem. 270: 2320-6. PMID: 7836465
10. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.  |  Kisen, GO., et al. 1993. Carcinogenesis. 14: 2501-5. PMID: 8269618
11. Autophagic proteolysis: control and specificity.  |  Blommaart, EF., et al. 1997. Histochem J. 29: 365-85. PMID: 9184851
12. Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.  |  Liang, XH., et al. 1998. J Virol. 72: 8586-96. PMID: 9765397

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BECN1/Beclin-1 Antibody (E-8) has 267 citations in a variety of scientific publications.

Primary antibodies like Anti-BECN1/Beclin-1 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BECN1/Beclin-1 Antibody (E-8) Specifications

Alternate Product Name: Beclin 1

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: Beclin 1

Epitope Species: human

Product Reactivity/Detection: Beclin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 267 citations in various scientific publications

Available Conjugates/Forms for BECN1/Beclin-1 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred BECN1/Beclin-1 antibody is BECN1/Beclin-1 Antibody (G-11): sc-48381.