Bcr Antibody (B-12): sc-28375

Bcr Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody, specifically raised against a deletion variant (amino acids 1-300 with deletion 17-219) of the human Bcr (Breakpoint Cluster Region) protein. The Bcr protein, also known as Renal carcinoma antigen NY-REN-26, is integral to cellular signaling pathways, functioning as a GTPase-activating protein (GAP) for Rac1 p21, which is essential for regulating cytoskeletal dynamics and cell migration. Structurally, Bcr comprises several functional domains, including the GAP domain, whose configuration is critical for its interaction with Rac1 and modulation of its activity, thereby maintaining proper cellular functions and preventing abnormal cell proliferation. The Bcr gene, located on chromosome 22, is famously involved in the formation of the Philadelphia chromosome through a translocation with the c-Abl proto-oncogene on chromosome 9, a genetic event present in approximately 90-95% of chronic myelogenous leukemia (CML) cases. This translocation results in the production of Bcr/Abl fusion proteins, such as p190 and p210, which possess constitutive tyrosine kinase activity that drives the uncontrolled growth of leukemic cells. Understanding the structure and function of Bcr is vital for elucidating the molecular mechanisms underlying CML and for developing targeted therapeutic strategies. Anti-Bcr antibody (B-12) effectively detects Bcr in mouse, rat, and human samples and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Additionally, Bcr monoclonal antibody (B-12) is available in multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® derivatives, offering versatility for diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcr Antibody (B-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcr Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcr Antibody (B-12) Specifications

Alternate Product Name: Bcr

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300 deletion 17-219

Epitope Target: Bcr

Epitope Species: human

Product Reactivity/Detection: Bcr

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Bcr Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred c-Abl antibody is c-Abl Antibody (24-11): sc-23.