Bcl-rambo Antibody (G-9): sc-390598

Bcl-rambo Antibody (G-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Bcl-rambo of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Bcl-rambo antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bcl-rambo, also known as BCL2L13 or MIL1, plays a crucial role in regulating apoptosis, a vital process for maintaining cellular homeostasis and eliminating damaged or unwanted cells. Located primarily in mitochondria, Bcl-rambo belongs to the Bcl-2 family and contains all four BH domains essential for function. Bcl-rambo features a unique C-terminal region with two tandem repeats preceding its membrane anchor, distinguishing Bcl-rambo from other Bcl-2 family members. This structural characteristic is significant because the membrane anchor region mediates pro-apoptotic activity, rather than Bcl-2 homology motifs. When overexpressed, Bcl-rambo induces apoptosis by promoting cytochrome c release from mitochondria, which subsequently activates caspase-3, a key player in the apoptotic cascade. Understanding Bcl-rambo′s function and mechanism is essential for elucidating its role in apoptosis and potential implications in various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bcl-rambo Antibody (G-9) References:

1. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension.  |  Kataoka, T., et al. 2001. J Biol Chem. 276: 19548-54. PMID: 11262395
2. Programmed cell death: alive and well in the new millennium.  |  Kaufmann, SH. and Hengartner, MO. 2001. Trends Cell Biol. 11: 526-34. PMID: 11719060
3. HIV-1 TAT-mediated protein transduction and subcellular localization using novel expression vectors.  |  Yang, Y., et al. 2002. FEBS Lett. 532: 36-44. PMID: 12459459
4. Overexpressed full-length human BCL2 extends the survival of baculovirus-infected Sf9 insect cells.  |  Alnemri, ES., et al. 1992. Proc Natl Acad Sci U S A. 89: 7295-9. PMID: 1502141
5. Effect of interaction of magnetic nanoparticles of Fe₃O₄ and artesunate on apoptosis of K562 cells.  |  Wang, Y., et al. 2011. Int J Nanomedicine. 6: 1185-92. PMID: 21822380
6. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.  |  Hockenbery, D., et al. 1990. Nature. 348: 334-6. PMID: 2250705
7. MNPs-Fe₃O₄mediates malignant Hematolpoectic cell apoptosis.  |  Li, YQ., et al. 2014. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 22: 1649-55. PMID: 25543491
8. Upregulation of miR-874-3p decreases cerebral ischemia/reperfusion injury by directly targeting BMF and BCL2L13.  |  Jiang, D., et al. 2019. Biomed Pharmacother. 117: 108941. PMID: 31200256
9. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.  |  Kerr, JF., et al. 1972. Br J Cancer. 26: 239-57. PMID: 4561027
10. Bcl-2 and the regulation of programmed cell death.  |  Reed, JC. 1994. J Cell Biol. 124: 1-6. PMID: 8294493
11. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
12. The Bcl-2 protein family: arbiters of cell survival.  |  Adams, JM. and Cory, S. 1998. Science. 281: 1322-6. PMID: 9735050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-rambo Antibody (G-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-rambo Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-rambo Antibody (G-9) Specifications

Alternate Product Name: Bcl-rambo

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 9-112

Epitope Target: Bcl-rambo

Epitope Species: human

Product Reactivity/Detection: Bcl-rambo

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Bcl-rambo Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin