BCAS3 Antibody (D-6): sc-365131
- BCAS3 Antibody (D-6) is a mouse monoclonal IgG1 κ BCAS3 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of BCAS3 of human origin
- BCAS3 Antibody (D-6) is recommended for detection of BCAS3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BCAS3 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BCAS3 Antibody (D-6) for WB applications. These reagents are now offered in bundles with BCAS3 Antibody (D-6) (see ordering information below).
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BCAS3 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects BCAS3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BCAS3 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BCAS3, also known as breast carcinoma amplified sequence 3, is a 913 amino acid protein that plays a significant role in breast cancer progression, making BCAS3 a critical target for cancer research. BCAS3 is regulated by estrogen receptor alpha (ERα) and is expressed in various tissues, particularly in malignant human brain lesions, where BCAS3 is often overexpressed and amplified in breast cancer cell lines. Structurally, BCAS3 features three WD40 repeat regions, a bromodomain, a rare zinc-finger motif, four probable DNA-binding domains, and two kinase-inducible phosphorylation domains, which are essential for BCAS3 function in cellular signaling and gene regulation. BCAS3 interacts with histone H3 and the histone acetyltransferase PCAF, indicating BCAS3 involvement in chromatin remodeling and transcriptional regulation. BCAS3 exhibits ERα transactivation activity by functioning as a coactivator with proteins such as PELP1 or MTA1, further underscoring BCAS3 importance in breast cancer biology. Amplification and translocation of BCAS3 gene with BCAS4 gene lead to the formation of a fusion transcript that is overexpressed in MCF-7 cells, highlighting BCAS3 potential as a biomarker and therapeutic target in breast cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BCAS3 Antibody (D-6) References:
- Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. | Bärlund, M., et al. 2002. Genes Chromosomes Cancer. 35: 311-7. PMID: 12378525
- Finding fusion genes resulting from chromosome rearrangement by analyzing the expressed sequence databases. | Hahn, Y., et al. 2004. Proc Natl Acad Sci U S A. 101: 13257-61. PMID: 15326299
- Multiple forms of genetic instability within a 2-Mb chromosomal segment of 3q26.3-q27 are associated with development of esophageal adenocarcinoma. | Lin, L., et al. 2006. Genes Chromosomes Cancer. 45: 319-31. PMID: 16320248
- MTA1, a transcriptional activator of breast cancer amplified sequence 3. | Gururaj, AE., et al. 2006. Proc Natl Acad Sci U S A. 103: 6670-5. PMID: 16617102
- Breast cancer-amplified sequence 3, a target of metastasis-associated protein 1, contributes to tamoxifen resistance in premenopausal patients with breast cancer. | Gururaj, AE., et al. 2006. Cell Cycle. 5: 1407-10. PMID: 16855396
- Estrogen induces expression of BCAS3, a novel estrogen receptor-alpha coactivator, through proline-, glutamic acid-, and leucine-rich protein-1 (PELP1). | Gururaj, AE., et al. 2007. Mol Endocrinol. 21: 1847-60. PMID: 17505058
- Human BCAS3 expression in embryonic stem cells and vascular precursors suggests a role in human embryogenesis and tumor angiogenesis. | Siva, K., et al. 2007. PLoS One. 2: e1202. PMID: 18030336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCAS3 Antibody (D-6) | sc-365131 | 200 µg/ml | $322.00 | |||
BCAS3 Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-529313 | 200 µg Ab; 10 µg BP | $361.00 | |||
BCAS3 Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-522142 | 200 µg Ab, 40 µg BP | $361.00 | |||
BCAS3 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-543448 | 200 µg Ab; 20 µg BP | $361.00 | |||
BCAS3 Antibody (D-6) AC | sc-365131 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BCAS3 Antibody (D-6) HRP | sc-365131 HRP | 200 µg/ml | $322.00 | |||
BCAS3 Antibody (D-6) FITC | sc-365131 FITC | 200 µg/ml | $336.00 | |||
BCAS3 Antibody (D-6) PE | sc-365131 PE | 200 µg/ml | $349.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 488 | sc-365131 AF488 | 200 µg/ml | $364.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 546 | sc-365131 AF546 | 200 µg/ml | $364.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 594 | sc-365131 AF594 | 200 µg/ml | $364.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 647 | sc-365131 AF647 | 200 µg/ml | $364.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 680 | sc-365131 AF680 | 200 µg/ml | $364.00 | |||
BCAS3 Antibody (D-6) Alexa Fluor® 790 | sc-365131 AF790 | 200 µg/ml | $364.00 |