Bax Antibody (B-9): sc-7480

Bax Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects Bax protein of mouse, rat, and human origin through applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry. Bax (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Bax protein plays a crucial role in the regulation of apoptosis, acting as a pro-apoptotic member of the Bcl-2 protein family. By promoting the release of cytochrome c from the mitochondria, Bax facilitates the activation of caspases, which are essential for the execution of programmed cell death. This function is particularly important in maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to cancer. Notably, Bax can form both homodimers and heterodimers with Bcl-2, a well-known anti-apoptotic protein, thereby influencing the balance between cell survival and death. Bax (B-9) antibody specificity ensures detection of Bax without cross-reactivity with Bcl-2, making this reagent a valuable tool for studying apoptotic pathways. Overexpression of Bax has been shown to accelerate apoptotic death in response to cytokine deprivation, highlighting its significance in cellular responses to stress and growth factor withdrawal.

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Bax Antibody (B-9) References:

1. Aldose reductase mediates endotoxin-induced production of nitric oxide and cytotoxicity in murine macrophages.  |  Ramana, KV., et al. 2007. Free Radic Biol Med. 42: 1290-302. PMID: 17382209
2. Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2.  |  Douglas, AE., et al. 2007. Protein Sci. 16: 695-703. PMID: 17384234
3. Expression of Bcl-xL, Bax, and p53 in primary tumors and lymph node metastases in oral squamous cell carcinoma.  |  Baltaziak, M., et al. 2006. Ann N Y Acad Sci. 1090: 18-25. PMID: 17384243
4. Alterations in mRNA expression of apoptosis-related genes BCL2, BAX, FAS, caspase-3, and the novel member BCL2L12 after treatment of human leukemic cell line HL60 with the antineoplastic agent etoposide.  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
5. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
6. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
7. The bcl-2 candidate proto-oncogene product is a 24-kilodalton integral-membrane protein highly expressed in lymphoid cell lines and lymphomas carrying the t(14;18) translocation.  |  Chen-Levy, Z., et al. 1989. Mol Cell Biol. 9: 701-10. PMID: 2651903
8. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells.  |  Vaux, DL., et al. 1988. Nature. 335: 440-2. PMID: 3262202
9. Physiological and pharmacological modulation of BAX.  |  Spitz, AZ. and Gavathiotis, E. 2022. Trends Pharmacol Sci. 43: 206-220. PMID: 34848097
10. BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps.  |  Maes, ME., et al. 2023. Mol Neurodegener. 18: 67. PMID: 37752598
11. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.  |  Bakhshi, A., et al. 1985. Cell. 41: 899-906. PMID: 3924412
12. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bax Antibody (B-9) has 1,530 citations in a variety of scientific publications.

Primary antibodies like Anti-Bax Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bax Antibody (B-9) Specifications

Alternate Product Name: Bax

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-171

Epitope Target: Bax

Epitope Species: mouse

Product Reactivity/Detection: Baxα and Baxβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,530 citations in various scientific publications

Available Conjugates/Forms for Bax Antibody (B-9): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin, TRITC: Rhodamine