Anti-Bax Antibody (B-9) recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-Bax Antibody (B-9): sc-7480

Anti-Bax Antibody (B-9) is rated 4.6 out of 5 by 81.
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Datasheets

    • Anti-Bax Antibody (B-9) is a mouse monoclonal IgG2b κ Bax antibody, cited in 1,305 publications, provided at 200 µg/ml
    • raised against amino acids 1-171 of Bax of mouse origin
    • Anti-Bax Antibody (B-9) is recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Bax Antibody (B-9) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bax (B-9): sc-7480.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Bax Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Bax Antibody (B-9) (see ordering information below).
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For flowcytometry can you share some articles proving BAX antibody and its isotypic control use? Also, can you please share of Flowcytometry staining protocol for this antibody and its isotypic control?

Asked by: RAMYAR
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi. May I know if your antibody is recombinant or hybridoma?

Asked by: Nghia
For more information on our Monoclonal Antibodies please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-05-12

 I run my sample on 10 % gel, I used 1/1000 in 5% BSA in TBS with  3h incubation, and I didn’t get any bands. Would you please advice me. 1)  What is the recommended dilution? The  dilute should be milk or BSA?  2) How long should I Incubate?What gel %?

Asked by: ddjoseph
Thank you for your question. We would recommend using the starting dilution for Western blot (1:200) and blocking your membrane with, and incubating the antibodies in 5% nonfat milk in TBST. For this protein we recommend a 12% gel. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2020-04-21

is there is a gift samples is available for BAX Bcl2?

Asked by: Dhana
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-03-10

I have three questions: 1) What gel percentage do you use to separate Baxα and Baxβ? 2) Does Bax Antibody (B-9): sc-7480 works for IF like the (6A7) clone: sc-23959? 3) If so, in human, in mouse cells or both species' cell lines?

Asked by: tiosulphat3
Thank you for your question. We are running a 15% SDS-PAGE gel for detection of Bax with this antibody. Both antibodies are recommended for IF on mouse, rat and human samples.
Answered by: Technical Support
Date published: 2020-03-10

Could you please suggest a suitable secondary antibody kit for this product as I am going to perform IHC (P) in Rat testis?

Asked by: Senthil
Thank you for your question. You can find our secondary reagents here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2020-03-10

can i use Bax Antibody (B-9) in goat? if yes what procedure do i follow?

Asked by: yana79
Thank you for the question. We recommended Bax Antibody (B-9) for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Unfortunately, we have not evaluated this antibody on samples derived from other animal species. Therefore, we are unable to predict cross-reactivity with the Bax protein from goat. If you were to try this, you might want to check and follow our protocols: https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support Europe
Date published: 2020-03-10

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2020-03-10
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Rated 5 out of 5 by from It works well! This antibody works well at 1:500 dilution, and no other bands were detected with high specificity.
Date published: 2021-09-17
Rated 5 out of 5 by from Ottimo funzionamento Anticorpo acquistato un anno fa e conservato correttamente a 4°C. Testato tramite Western Blotting su cellule provenienti da topi CD1. Ottima presenza della banda specifica.
Date published: 2021-05-28
Rated 5 out of 5 by from Great antibody for WB This primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of 5 by from Good for WB This antibody worked well for western blotting. I could detect a specific band at approximately 20 kDa.
Date published: 2019-03-19
Rated 5 out of 5 by from Great purchase! Fast delivery! Everything is great. Highly recommend!
Date published: 2019-03-07
Rated 5 out of 5 by from a good antibody for WB This works well for mouse Bax western blot (1 : 1000).
Date published: 2018-11-02
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from 效果比较棒 近期刚使用了这支抗体,按照说明书给的稀释浓度液按照1:800的稀释比例稀释,利用乳腺癌细胞发出的条带效果不错,符合预期。
Date published: 2018-04-21
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