Anti-Bax Antibody (B-9): sc-7480
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- Anti-Bax Antibody (B-9) is a mouse monoclonal IgG2b κ Bax antibody, cited in 1,305 publications, provided at 200 µg/ml
- raised against amino acids 1-171 of Bax of mouse origin
- Anti-Bax Antibody (B-9) is recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Bax Antibody (B-9) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to TRITC, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bax (B-9): sc-7480.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Bax Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Bax Antibody (B-9) (see ordering information below).
QUICK LINKS
SEE ALSO...
- ChemCruz™ Chemical Bax Activators for functional analysis of cellular responses to Bax
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
For flowcytometry can you share some articles proving BAX antibody and its isotypic control use? Also, can you please share of Flowcytometry staining protocol for this antibody and its isotypic control?
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Date published: 2021-07-22
Hi. May I know if your antibody is recombinant or hybridoma?
For more information on our Monoclonal Antibodies please contact Technical Service.
Date published: 2021-05-12
I run my sample on 10 % gel, I used 1/1000 in 5% BSA in TBS with 3h incubation, and I didn’t get any bands. Would you please advice me. 1) What is the recommended dilution? The dilute should be milk or BSA? 2) How long should I Incubate?What gel %?
Thank you for your question. We would recommend using the starting dilution for Western blot (1:200) and blocking your membrane with, and incubating the antibodies in 5% nonfat milk in TBST. For this protein we recommend a 12% gel. A link to our recommended protocol is below.
https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Date published: 2020-04-21
is there is a gift samples is available for BAX Bcl2?
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Date published: 2020-03-10
I have three questions: 1) What gel percentage do you use to separate Baxα and Baxβ? 2) Does Bax Antibody (B-9): sc-7480 works for IF like the (6A7) clone: sc-23959? 3) If so, in human, in mouse cells or both species' cell lines?
Thank you for your question. We are running a 15% SDS-PAGE gel for detection of Bax with this antibody. Both antibodies are recommended for IF on mouse, rat and human samples.
Date published: 2020-03-10
Could you please suggest a suitable secondary antibody kit for this product as I am going to perform IHC (P) in Rat testis?
Thank you for your question. You can find our secondary reagents here:
https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Date published: 2020-03-10
can i use Bax Antibody (B-9) in goat? if yes what procedure do i follow?
Thank you for the question. We recommended Bax Antibody (B-9) for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Unfortunately, we have not evaluated this antibody on samples derived from other animal species. Therefore, we are unable to predict cross-reactivity with the Bax protein from goat. If you were to try this, you might want to check and follow our protocols: https://www.scbt.com/scbt/resources/protocols
Date published: 2020-03-10
I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Date published: 2020-03-10
Rated 5 out of
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tarring from
It works well!
This antibody works well at 1:500 dilution, and no other bands were detected with high specificity.
Date published: 2021-09-17
Rated 5 out of
5
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Anonymous from
Ottimo funzionamento
Anticorpo acquistato un anno fa e conservato correttamente a 4°C. Testato tramite Western Blotting su cellule provenienti da topi CD1. Ottima presenza della banda specifica.
Date published: 2021-05-28
Rated 5 out of
5
by
HL Yang from
Great antibody for WB
This primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of
5
by
Jennifer Chen from
Good for WB
This antibody worked well for western blotting. I could detect a specific band at approximately 20 kDa.
Date published: 2019-03-19
Rated 5 out of
5
by
amogilevtseva from
Great purchase!
Fast delivery! Everything is great. Highly recommend!
Date published: 2019-03-07
Rated 5 out of
5
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shinomako from
a good antibody for WB
This works well for mouse Bax western blot (1 : 1000).
Date published: 2018-11-02
Rated 5 out of
5
by
Hlyang from
Great product!!
It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of
5
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Drzhao from
效果比较棒
近期刚使用了这支抗体，按照说明书给的稀释浓度液按照1：800的稀释比例稀释，利用乳腺癌细胞发出的条带效果不错，符合预期。
Date published: 2018-04-21