BAT1/DDX39 Antibody (H-6): sc-271395

BAT1/DDX39 Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects BAT1/DDX39 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BAT1/DDX39 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BAT1 protein, also known as spliceosome RNA helicase BAT1, DEAD-box protein UAP56, or ATP-dependent RNA helicase p47, plays a crucial role in mRNA splicing and nuclear export, functioning as a splicing factor that mediates the initial ATP-dependent step of spliceosome assembly. BAT1 interacts with mRNA export factor ALY and associates with transcription elongation factor THO proteins to form TREX complex, which is essential for efficient export of mRNA from the nucleus. BAT1 is involved in formation of exon junction complex, highlighting its importance in post-transcriptional regulation. DDX39 protein, closely related to BAT1, is localized in the nucleus and is expressed in various tissues, including lung, brain, kidney, spleen, thymus, and salivary gland. DDX39 is implicated in pre-mRNA splicing and mRNA export, with expression being upregulated in lung squamous cell carcinoma, suggesting a potential role in tumorigenesis. BAT1/DDX39 (H-6) monoclonal antibody′s availability in multiple formats makes this reagent a versatile tool for researchers studying intricate processes of RNA metabolism and implications in health and disease.

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BAT1/DDX39 Antibody (H-6) References:

1. Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis.  |  Momose, F., et al. 2001. J Virol. 75: 1899-908. PMID: 11160689
2. Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly.  |  Luo, ML., et al. 2001. Nature. 413: 644-7. PMID: 11675789
3. Polymorphisms at positions -22 and -348 in the promoter of the BAT1 gene affect transcription and the binding of nuclear factors.  |  Price, P., et al. 2004. Hum Mol Genet. 13: 967-74. PMID: 15028669
4. Crystal structure of the human ATP-dependent splicing and export factor UAP56.  |  Shi, H., et al. 2004. Proc Natl Acad Sci U S A. 101: 17628-33. PMID: 15585580
5. Homolog of BRCA2-interacting Dss1p and Uap56p link Mlo3p and Rae1p for mRNA export in fission yeast.  |  Thakurta, AG., et al. 2005. EMBO J. 24: 2512-23. PMID: 15990877
6. BAT1, a putative anti-inflammatory gene, is associated with chronic Chagas cardiomyopathy.  |  Ramasawmy, R., et al. 2006. J Infect Dis. 193: 1394-9. PMID: 16619187
7. Nuclear localization of poly(A)+ mRNA following siRNA reduction of expression of the mammalian RNA helicases UAP56 and URH49.  |  Kapadia, F., et al. 2006. Gene. 384: 37-44. PMID: 16949217
8. DDX39, upregulated in lung squamous cell cancer, displays RNA helicase activities and promotes cancer cell growth.  |  Sugiura, T., et al. 2007. Cancer Biol Ther. 6: 957-64. PMID: 17548965
9. DDX39 as a predictor of clinical prognosis and immune checkpoint therapy efficacy in patients with clear cell renal cell carcinoma.  |  Bao, Y., et al. 2021. Int J Biol Sci. 17: 3158-3172. PMID: 34421357
10. CASP1 Gene Polymorphisms and BAT1-NFKBIL-LTA-CASP1 Gene-Gene Interactions Are Associated with Restenosis after Coronary Stenting.  |  Vargas-Alarcón, G., et al. 2022. Biomolecules. 12: PMID: 35740890
11. Novel role for Ddx39 in differentiation and telomere length regulation of embryonic stem cells.  |  Nai, S., et al. 2024. Cell Death Differ. 31: 1534-1544. PMID: 39107495
12. U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction.  |  Fleckner, J., et al. 1997. Genes Dev. 11: 1864-72. PMID: 9242493

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BAT1/DDX39 Antibody (H-6) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-BAT1/DDX39 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BAT1/DDX39 Antibody (H-6) Specifications

Alternate Product Name: BAT1/DDX39

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 231-428

Epitope Target: BAT1

Epitope Species: human

Product Reactivity/Detection: BAT1 and DDX39

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for BAT1/DDX39 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent