Barttin Antibody (A-1): sc-271867

Barttin Antibody (A-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects Barttin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Barttin antibody (A-1) is available as the non-conjugated form. The BSND gene encodes Barttin, a protein characterized by two putative transmembrane alpha helices, which play a crucial role in functioning as a b subunit for chloride channels CLCKNA and CLCKNB. This interaction is vital for proper renal salt reabsorption and potassium recycling in the inner ear, highlighting Barttin′s importance in maintaining electrolyte balance and auditory function. Barttin (A-1) antibody detects expression predominantly in the thin limb and thick ascending limb of the loop of Henle in the kidney, as well as in the dark cells of the inner ear, where Barttin co-localizes with chloride channels in basolateral membranes of renal tubules and potassium-secreting epithelia. Mutations in BSND are associated with Bartter syndrome, a genetic disorder leading to hypokalemia, metabolic alkalosis, and sensorineural deafness, underscoring Barttin′s critical role in both renal and auditory health. Mutations in either CLCKNB or Barttin can compromise channel functionality, affecting currents through heteromeric channels and emphasizing the intricate relationship between Barttin and chloride channel activity.

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Barttin Antibody (A-1) References:

1. Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.  |  Estévez, R., et al. 2001. Nature. 414: 558-61. PMID: 11734858
2. Atypical Bartter syndrome with sensorineural deafness with G47R mutation of the beta-subunit for ClC-Ka and ClC-Kb chloride channels, barttin.  |  Miyamura, N., et al. 2003. J Clin Endocrinol Metab. 88: 781-6. PMID: 12574213
3. Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide.  |  Wolf, K., et al. 2003. Pflugers Arch. 446: 665-71. PMID: 12759757
4. Investigations of pharmacologic properties of the renal CLC-K1 chloride channel co-expressed with barttin by the use of 2-(p-Chlorophenoxy)propionic acid derivatives and other structurally unrelated chloride channels blockers.  |  Liantonio, A., et al. 2004. J Am Soc Nephrol. 15: 13-20. PMID: 14694153
5. Regulation of CLC-Ka/barttin by the ubiquitin ligase Nedd4-2 and the serum- and glucocorticoid-dependent kinases.  |  Embark, HM., et al. 2004. Kidney Int. 66: 1918-25. PMID: 15496163
6. Renal tubular transport and the genetic basis of hypertensive disease.  |  Lang, F., et al. 2005. Clin Exp Nephrol. 9: 91-9. PMID: 15980941
7. How Bartter's and Gitelman's syndromes, and Dent's disease have provided important insights into the function of three renal chloride channels: ClC-Ka/b and ClC-5.  |  Briet, M., et al. 2006. Nephron Physiol. 103: p7-13. PMID: 16352917
8. Barttin activates ClC-K channel function by modulating gating.  |  Fischer, M., et al. 2010. J Am Soc Nephrol. 21: 1281-9. PMID: 20538786
9. Tryptophan Scanning Mutagenesis Identifies the Molecular Determinants of Distinct Barttin Functions.  |  Wojciechowski, D., et al. 2015. J Biol Chem. 290: 18732-43. PMID: 26063802
10. Barttin Regulates the Subcellular Localization and Posttranslational Modification of Human Cl-/H+ Antiporter ClC-5.  |  Wojciechowski, D., et al. 2018. Front Physiol. 9: 1490. PMID: 30405442
11. DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels.  |  Gorinski, N., et al. 2020. J Biol Chem. 295: 5970-5983. PMID: 32184353
12. Regulation of ClC-K/barttin by endocytosis influences distal convoluted tubule hyperplasia.  |  Mayayo-Vallverdú, C., et al. 2024. J Physiol. 602: 4291-4307. PMID: 39106251

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Barttin Antibody (A-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Barttin Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Barttin Antibody (A-1) Specifications

Alternate Product Name: Barttin

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 89-117

Epitope Target: Barttin

Epitope Species: human

Product Reactivity/Detection: Barttin isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Barttin Antibody (A-1): P: Blocking Peptide