Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BANP CRISPR/Cas9 KO Plasmid (h): sc-412401
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BANP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BANP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BANP Antibody (1524CT337.6.32): sc-517314
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BANP CRISPR/Cas9 KO Plasmid (h) | sc-412401 | 20 µg | $397.00 |
Overview
BANP (BTG3-associated nuclear protein), also known as SMAR1, is a nuclear DNA-binding factor implicated in chromatin organization and transcriptional control at matrix attachment regions. It has been linked to regulation of cell-cycle progression, checkpoint responses, and maintenance of genome stability through coordination of transcriptional programs and DNA damage–associated processes. BANP activity intersects with chromatin remodeling and tumor suppressor–associated signaling networks, influencing proliferation and stress-induced gene expression. Dysregulation of BANP expression or function has been associated with altered growth control and genome integrity phenotypes relevant to cancer biology and related cellular models.
BANP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BANP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BANP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BANP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BANP protein expression.
This CRISPR knockout system enables efficient generation of BANP-deficient cell models for investigation of BANP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BANP exon(s) critical for BANP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BANP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BANP CRISPR/Cas9 KO Plasmid (h) and BANP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BANP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BANP HDR Plasmid (h) and BANP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BANP homology arms to support homology-directed repair at defined BANP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.