Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BANK1 CRISPR/Cas9 KO Plasmid (h): sc-405620
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BANK1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BANK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BANK1 Antibody (F-8): sc-393611
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BANK1 CRISPR/Cas9 KO Plasmid (h) | sc-405620 | 20 µg | $397.00 |
Overview
BANK1 (B-cell scaffold protein with ankyrin repeats 1) is a B lymphocyte–enriched adaptor protein that integrates signaling downstream of the B cell antigen receptor and innate immune receptors to shape activation, calcium mobilization, and transcriptional outputs. Through scaffold-mediated assembly of signaling complexes, BANK1 influences pathways linked to NF-κB and MAPK signaling and can modulate cytokine responses and B cell differentiation programs. Genetic and expression studies associate BANK1 with immune dysregulation and susceptibility to autoimmune phenotypes, including systemic lupus erythematosus, supporting its relevance to inflammatory signaling networks. As a regulator of B cell signaling architecture, BANK1 is frequently studied in contexts such as antigen-driven activation, tolerance checkpoints, and cross-talk with interferon-related responses.
BANK1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BANK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BANK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BANK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BANK1 protein expression.
This CRISPR knockout system enables efficient generation of BANK1-deficient cell models for investigation of BANK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BANK1 exon(s) critical for BANK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BANK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BANK1 CRISPR/Cas9 KO Plasmid (h) and BANK1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BANK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BANK1 HDR Plasmid (h) and BANK1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BANK1 homology arms to support homology-directed repair at defined BANK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.