Bag-3 Antibody (19): sc-136467

Bag-3 Antibody (19) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bag-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-Bag-3 antibody (19) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). Bag-3 protein, also known as Bcl-2-associated athanogene 3 or BIS, is a 575 amino acid protein characterized by a C-terminal Bag domain and two N-terminal WW domains. This unique structure allows Bag-3 to interact with the ATPase domain of HSP 70, effectively inhibiting HSP 70′s chaperone activity and promoting the release of HSP 70-bound substrates. Such regulatory function maintains cellular protein homeostasis and prevents misfolded protein accumulation, which can lead to cellular stress and apoptosis. Bag-3′s role in cell cycle control and anti-apoptotic properties suggest potential contributions to tumor progression, making Bag-3 an important target for cancer research. Bag-3 gene is located on human chromosome 10, a region containing over 1,200 genes and representing nearly 4.5% of the human genome, highlighting Bag-3′s significance in various biological processes.

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Bag-3 Antibody (19) References:

1. The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.  |  Liao, Q., et al. 2001. FEBS Lett. 503: 151-7. PMID: 11513873
2. CAIR-1/BAG-3 abrogates heat shock protein-70 chaperone complex-mediated protein degradation: accumulation of poly-ubiquitinated Hsp90 client proteins.  |  Doong, H., et al. 2003. J Biol Chem. 278: 28490-500. PMID: 12750378
3. The antiapoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand.  |  Chiappetta, G., et al. 2007. J Clin Endocrinol Metab. 92: 1159-63. PMID: 17164298
4. BAG3 regulates motility and adhesion of epithelial cancer cells.  |  Iwasaki, M., et al. 2007. Cancer Res. 67: 10252-9. PMID: 17974966
5. Transcriptional upregulation of BAG3 upon proteasome inhibition.  |  Wang, HQ., et al. 2008. Biochem Biophys Res Commun. 365: 381-5. PMID: 17996194
6. HspB8 chaperone activity toward poly(Q)-containing proteins depends on its association with Bag3, a stimulator of macroautophagy.  |  Carra, S., et al. 2008. J Biol Chem. 283: 1437-1444. PMID: 18006506
7. HspB8 and Bag3: a new chaperone complex targeting misfolded proteins to macroautophagy.  |  Carra, S., et al. 2008. Autophagy. 4: 237-9. PMID: 18094623
8. Bag3 gene expression is regulated by heat shock factor 1.  |  Franceschelli, S., et al. 2008. J Cell Physiol. 215: 575-7. PMID: 18286539
9. Activation of BAG3 by Egr-1 in response to FGF-2 in neuroblastoma cells.  |  Gentilella, A., et al. 2008. Oncogene. 27: 5011-8. PMID: 18469860
10. Bag-3 expression is involved in pathogenesis and progression of colorectal carcinomas.  |  Yang, X., et al. 2013. Histol Histopathol. 28: 1147-56. PMID: 23553442
11. On the origin of BAG(3) and its consequences for an expansion of BAG3's role in protein homeostasis.  |  Baeken, MW. and Behl, C. 2022. J Cell Biochem. 123: 102-114. PMID: 33942360
12. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators.  |  Takayama, S., et al. 1999. J Biol Chem. 274: 781-6. PMID: 9873016

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bag-3 Antibody (19) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Bag-3 Antibody (19) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bag-3 Antibody (19) Specifications

Alternate Product Name: Bag-3

Product Clone Name: 19

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 478-575

Epitope Target: Bag-3

Epitope Species: human

Product Reactivity/Detection: Bag-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Bag-3 Antibody (19): AC: Agarose, HRP: Horseradish Peroxidase