Bag-2 Antibody (C-6): sc-390107
- Bag-2 Antibody (C-6) is a mouse monoclonal IgG1 κ Bag-2 antibody provided at 200 µg/ml
- raised against amino acids 1-211 representing full length Bag-2 of human origin
- Bag-2 Antibody (C-6) is recommended for detection of Bag-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Bag-2 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Bag-2 Antibody (C-6) for WB applications. These reagents are now offered in bundles with Bag-2 Antibody (C-6) (see ordering information below).
Bag-2 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bag-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bag-2 (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bag-2 protein, also known as Bcl-2-associated athanogene 2, plays a crucial role in cellular stress responses by functioning as a co-chaperone for HSC 70, a heat shock protein that assists in protein folding and protection against misfolded proteins. This interaction is vital for maintaining cellular homeostasis, particularly under stress conditions, as Bag-2 helps regulate apoptosis and prevent damaged protein accumulation. Bag-2 is an evolutionarily conserved cytoplasmic protein containing putative N-terminal phosphorylation sites, which may influence activity and interactions. Notably, Bag-2 functions as a key component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex, where Bag-2 disrupts CHIP-mediated ubiquitylation by interfering with the association between CHIP and ubiquitin-conjugating enzyme, thereby modulating protein degradation pathways. Bag-2′s ability to regulate these processes underscores importance in cellular function and potential implications in various diseases, including cancer and neurodegenerative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Bag-2 Antibody (C-6) References:
- Proteomic identification of Bcl2-associated athanogene 2 as a novel MAPK-activated protein kinase 2 substrate. | Ueda, K., et al. 2004. J Biol Chem. 279: 41815-21. PMID: 15271996
- Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells. | Götz, R., et al. 2005. Nat Neurosci. 8: 1169-78. PMID: 16116448
- Regulation of the cytoplasmic quality control protein degradation pathway by BAG2. | Dai, Q., et al. 2005. J Biol Chem. 280: 38673-81. PMID: 16169850
- BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP. | Arndt, V., et al. 2005. Mol Biol Cell. 16: 5891-900. PMID: 16207813
- Affinity purification reveals the association of WD40 protein constitutive photomorphogenic 1 with the hetero-oligomeric TCP-1 chaperonin complex in mammalian cells. | Yi, C., et al. 2006. Int J Biochem Cell Biol. 38: 1076-83. PMID: 16497536
- A genomewide analysis of genes for the heat shock protein 70 chaperone system in the ascidian Ciona intestinalis. | Wada, S., et al. 2006. Cell Stress Chaperones. 11: 23-33. PMID: 16572726
- To be, or not to be--molecular chaperones in protein degradation. | Arndt, V., et al. 2007. Cell Mol Life Sci. 64: 2525-41. PMID: 17565442
- Co-chaperone FKBP38 promotes HERG trafficking. | Walker, VE., et al. 2007. J Biol Chem. 282: 23509-16. PMID: 17569659
- An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. | Takayama, S., et al. 1999. J Biol Chem. 274: 781-6. PMID: 9873016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bag-2 Antibody (C-6) | sc-390107 | 200 µg/ml | $316.00 | |||
Bag-2 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-530061 | 200 µg Ab; 10 µg BP | $354.00 | |||
Bag-2 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-523405 | 200 µg Ab, 40 µg BP | $354.00 | |||
Bag-2 Antibody (C-6): m-IgG1 BP-HRP Bundle | sc-543849 | 200 µg Ab; 20 µg BP | $354.00 | |||
Bag-2 Antibody (C-6) AC | sc-390107 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Bag-2 Antibody (C-6) HRP | sc-390107 HRP | 200 µg/ml | $316.00 | |||
Bag-2 Antibody (C-6) FITC | sc-390107 FITC | 200 µg/ml | $330.00 | |||
Bag-2 Antibody (C-6) PE | sc-390107 PE | 200 µg/ml | $343.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 488 | sc-390107 AF488 | 200 µg/ml | $357.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 546 | sc-390107 AF546 | 200 µg/ml | $357.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 594 | sc-390107 AF594 | 200 µg/ml | $357.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 647 | sc-390107 AF647 | 200 µg/ml | $357.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 680 | sc-390107 AF680 | 200 µg/ml | $357.00 | |||
Bag-2 Antibody (C-6) Alexa Fluor® 790 | sc-390107 AF790 | 200 µg/ml | $357.00 |