Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BACE2 CRISPR/Cas9 KO Plasmid (m): sc-425013
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BACE2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BACE2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BACE2 Antibody (H-3): sc-271212
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BACE2 CRISPR/Cas9 KO Plasmid (m) | sc-425013 | 20 µg | $397.00 |
Overview
Bace2 encodes the mouse β-site APP-cleaving enzyme 2 (BACE2), a type I transmembrane aspartyl protease that participates in regulated intramembrane proteolysis of select substrates in the secretory and endosomal pathways. BACE2 activity influences proteostasis and membrane protein processing, intersecting with vesicular trafficking, lysosomal turnover, and cellular stress responses linked to neuronal and metabolic physiology. In the central nervous system, BACE2 has been studied in the context of amyloid precursor protein family processing and peptide generation, informing research on pathways implicated in neurodegeneration. In peripheral tissues, including pancreatic islets, BACE2-associated substrate cleavage has been connected to β-cell function, making it relevant for mechanistic studies of metabolic disease biology.
BACE2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Bace2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Bace2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Bace2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BACE2 protein expression.
This CRISPR knockout system enables efficient generation of Bace2-deficient cell models for investigation of BACE2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Bace2 exon(s) critical for BACE2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Bace2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BACE2 CRISPR/Cas9 KO Plasmid (m) and BACE2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Bace2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BACE2 HDR Plasmid (m) and BACE2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Bace2 homology arms to support homology-directed repair at defined Bace2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.