BACE Antibody (61-3E7): sc-33711

BACE Antibody (61-3E7) is a mouse monoclonal IgG1 kappa light chain antibody that detects BACE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-BACE antibody (61-3E7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BACE protein, also known as Beta-Site APP Cleaving Enzyme 1, plays a crucial role in the pathogenesis of Alzheimer′s disease by cleaving amyloid precursor protein (APP) to produce amyloid β-peptide (Aβ), a major component of amyloid plaques found in the brains of Alzheimer′s patients. The cleavage of APP by BACE is a critical step in the formation of these plaques, which are associated with neurodegeneration and cognitive decline. BACE is predominantly localized within the Golgi apparatus and endosomes, which is significant as BACE activity is regulated by the cellular environment and trafficking pathways. Understanding the localization and function of BACE is essential for developing therapeutic strategies aimed at modulating BACE activity to prevent or treat Alzheimer′s disease. BACE and related protein, Asp1, are widely expressed in human tissues, with highest expression levels found in the pancreas, highlighting BACE importance in both neurological and metabolic processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BACE Antibody (61-3E7) References:

1. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE.  |  Vassar, R., et al. 1999. Science. 286: 735-41. PMID: 10531052
2. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.  |  Yan, R., et al. 1999. Nature. 402: 533-7. PMID: 10591213
3. Identification of a novel aspartic protease (Asp 2) as beta-secretase.  |  Hussain, I., et al. 1999. Mol Cell Neurosci. 14: 419-27. PMID: 10656250
4. A pathogenic mutation for probable Alzheimer's disease in the APP gene at the N-terminus of beta-amyloid.  |  Mullan, M., et al. 1992. Nat Genet. 1: 345-7. PMID: 1302033
5. Human BACE forms dimers and colocalizes with APP.  |  Schmechel, A., et al. 2004. J Biol Chem. 279: 39710-7. PMID: 15247262
6. Apo and inhibitor complex structures of BACE (beta-secretase).  |  Patel, S., et al. 2004. J Mol Biol. 343: 407-16. PMID: 15451669
7. Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy.  |  von Arnim, CA., et al. 2004. J Cell Sci. 117: 5437-45. PMID: 15466887
8. BACE overexpression alters the subcellular processing of APP and inhibits Abeta deposition in vivo.  |  Lee, EB., et al. 2005. J Cell Biol. 168: 291-302. PMID: 15642747
9. Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease.  |  Goate, A., et al. 1991. Nature. 349: 704-6. PMID: 1671712
10. Application of fragment-based NMR screening, X-ray crystallography, structure-based design, and focused chemical library design to identify novel microM leads for the development of nM BACE-1 (beta-site APP cleaving enzyme 1) inhibitors.  |  Wang, YS., et al. 2010. J Med Chem. 53: 942-50. PMID: 20043700
11. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor.  |  Kang, J., et al. Nature. 325: 733-6. PMID: 2881207
12. The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease.  |  Selkoe, DJ. 1998. Trends Cell Biol. 8: 447-53. PMID: 9854312

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BACE Antibody (61-3E7) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-BACE Antibody (61-3E7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BACE Antibody (61-3E7) Specifications

Alternate Product Name: BACE1

Product Clone Name: 61-3E7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: BACE

Epitope Species: human

Product Reactivity/Detection: BACE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for BACE Antibody (61-3E7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin