Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BAALC CRISPR/Cas9 KO Plasmid (h): sc-405852
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAALC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BAALC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BAALC Antibody (H-12): sc-515606
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAALC CRISPR/Cas9 KO Plasmid (h) | sc-405852 | 20 µg | $397.00 |
Overview
BAALC (brain and acute leukemia, cytoplasmic) encodes a cytoplasmic protein enriched in hematopoietic and neurodevelopmental contexts and implicated in regulation of progenitor cell state, including aspects of proliferation and differentiation. In myeloid lineages, BAALC expression is frequently used as a molecular correlate of immature transcriptional programs and altered signaling outputs, intersecting with pathways that shape cell-cycle progression, survival, and lineage commitment. Dysregulated BAALC levels have been associated with acute leukemias, where it is studied as part of broader gene expression networks linked to leukemogenesis and disease heterogeneity. These features make BAALC a useful node for dissecting mechanisms of stem/progenitor maintenance and aberrant hematopoietic signaling in human cellular models.
BAALC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BAALC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BAALC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BAALC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BAALC protein expression.
This CRISPR knockout system enables efficient generation of BAALC-deficient cell models for investigation of BAALC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BAALC exon(s) critical for BAALC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BAALC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BAALC CRISPR/Cas9 KO Plasmid (h) and BAALC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BAALC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BAALC HDR Plasmid (h) and BAALC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BAALC homology arms to support homology-directed repair at defined BAALC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.