B7-2 Antibody (D-6): sc-28347

B7-2 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects B7-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). B7-2 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. B7-2, also known as CD86, plays a crucial role in immune response by providing essential costimulatory signals necessary for T cell activation. B7-2 is primarily located on antigen-presenting cells, where B7-2 interacts with T cell receptors such as CD28 and CTLA-4, facilitating T cell activation and proliferation. This interaction ensures proper T cell stimulation for pathogen response. B7-2 regulates immune tolerance and influences immune response outcomes, making B7-2 a significant target for therapeutic interventions in cancer and autoimmune disorders.

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B7-2 Antibody (D-6) References:

1. B7-1 and B7-2 co-stimulatory molecules are required for mercury-induced autoimmunity.  |  Bagenstose, LM., et al. 2002. Clin Exp Immunol. 127: 12-9. PMID: 11882027
2. B7-1 (CD80) and B7-2 (CD 86) expression in human tubular epithelial cells in vivo and in vitro.  |  Niemann-Masanek, U., et al. 2002. Nephron. 92: 542-56. PMID: 12372936
3. Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy.  |  Schwartz, RH. 1992. Cell. 71: 1065-8. PMID: 1335362
4. Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.  |  Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
5. Toxoplasma gondii induces B7-2 expression through activation of JNK signal transduction.  |  Morgado, P., et al. 2011. Infect Immun. 79: 4401-12. PMID: 21911468
6. Potential roles of the B7 and CD28 receptor families in autoimmunity and immune evasion.  |  Harlan, DM., et al. 1995. Clin Immunol Immunopathol. 75: 99-111. PMID: 7535672
7. Identification of residues in the V domain of CD80 (B7-1) implicated in functional interactions with CD28 and CTLA4.  |  Fargeas, CA., et al. 1995. J Exp Med. 182: 667-75. PMID: 7544393
8. Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28.  |  Peach, RJ., et al. 1995. J Biol Chem. 270: 21181-7. PMID: 7545666
9. A novel receptor involved in T-cell activation.  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
10. CTLA4 mediates antigen-specific apoptosis of human T cells.  |  Gribben, JG., et al. 1995. Proc Natl Acad Sci U S A. 92: 811-5. PMID: 7846057
11. Heterogeneous effects of B7-1 and B7-2 in the induction of both protective and therapeutic anti-tumor immunity against different mouse tumors.  |  Martin-Fontecha, A., et al. 1996. Eur J Immunol. 26: 1851-9. PMID: 8765031
12. B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation.  |  Borriello, F., et al. 1997. Immunity. 6: 303-13. PMID: 9075931

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-B7-2 Antibody (D-6) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-B7-2 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

B7-2 Antibody (D-6) Specifications

Alternate Product Name: CD86

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 24-223

Epitope Target: B7-2

Epitope Species: human

Product Reactivity/Detection: B7-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for B7-2 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin