B42 Antibody (E-2): sc-377101
- B42 Antibody (E-2) is a mouse monoclonal IgG1 κ B42 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 57-93 within an internal region of the transcriptional activation domain of B42
- B42 Antibody (E-2) is recommended for detection of B42 fusion proteins by WB, IP, IF and ELISA
- Anti-B42 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for B42 Antibody (E-2) for WB applications. These reagents are now offered in bundles with B42 Antibody (E-2) (see ordering information below).
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SEE ALSO...
B42 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects B42 protein of Saccharomyces cerevisiae by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). B42 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. B42 protein serves as a transcriptional activation domain, playing a crucial role in the two-hybrid system, a powerful method for studying protein-protein interactions. This system utilizes genetically engineered yeast or bacteria, where a ′bait′ protein is fused to a DNA-binding domain, and a ′prey′ protein is linked to a transcriptional activation domain, resulting in the activation of reporter genes upon their interaction. B42 protein is particularly valuable as an alternative activation domain, especially in configurations using the LexA DNA-binding domain, enhancing the versatility of the two-hybrid system across various organisms. B42 protein′s ability to facilitate robust transcriptional activation is essential for accurately mapping interactomes and conducting proteomics studies, as this function ensures specific protein localization within the cell and allows for detailed exploration of cellular functions and interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
B42 Antibody (E-2) References:
- GAL4 fusion vectors for expression in yeast or mammalian cells. | Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
- The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. | Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
- Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation. | Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
- Activators and targets. | Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
- A novel genetic system to detect protein-protein interactions. | Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
- A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. | Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
- Deletion analysis of GAL4 defines two transcriptional activating segments. | Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B42 Antibody (E-2) | sc-377101 | 200 µg/ml | $322.00 | |||
B42 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-527580 | 200 µg Ab; 10 µg BP | $361.00 | |||
B42 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-532953 | 200 µg Ab; 20 µg BP | $361.00 | |||
B42 Antibody (E-2) AC | sc-377101 AC | 500 µg/ml, 25% agarose | $424.00 | |||
B42 Antibody (E-2) HRP | sc-377101 HRP | 200 µg/ml | $322.00 | |||
B42 Antibody (E-2) FITC | sc-377101 FITC | 200 µg/ml | $336.00 | |||
B42 Antibody (E-2) PE | sc-377101 PE | 200 µg/ml | $349.00 | |||
B42 Antibody (E-2) Alexa Fluor® 488 | sc-377101 AF488 | 200 µg/ml | $364.00 | |||
B42 Antibody (E-2) Alexa Fluor® 546 | sc-377101 AF546 | 200 µg/ml | $364.00 | |||
B42 Antibody (E-2) Alexa Fluor® 594 | sc-377101 AF594 | 200 µg/ml | $364.00 | |||
B42 Antibody (E-2) Alexa Fluor® 647 | sc-377101 AF647 | 200 µg/ml | $364.00 | |||
B42 Antibody (E-2) Alexa Fluor® 680 | sc-377101 AF680 | 200 µg/ml | $364.00 | |||
B42 Antibody (E-2) Alexa Fluor® 790 | sc-377101 AF790 | 200 µg/ml | $364.00 | |||
B42 (E-2) Neutralizing Peptide | sc-377101 P | 100 µg/0.5 ml | $69.00 |