Date published: 2025-10-17

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B23/Nucleophosmin Antibody (3F291): sc-70392

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Datasheets
  • B23/Nucleophosmin Antibody (3F291) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
  • raised against a recombinant protein corresponding to the N-terminus of B23 of human origin
  • recommended for detection of B23 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See B23/Nucleophosmin (E-3): sc-271737 for B23/Nucleophosmin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for B23/Nucleophosmin Antibody (3F291) for WB and IHC(P) applications. These reagents are now offered in bundles with B23/Nucleophosmin Antibody (3F291) (see ordering information below).

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B23 Antibody (3F291) is a mouse monoclonal IgG1 antibody that detects B23 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. B23, also known as nucleophosmin, plays a crucial role in ribosomal assembly and the transport of ribosomal RNA, making B23 essential for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in the maturation of ribosomal subunits, and proper localization is vital for maintaining efficient ribosome biogenesis. Additionally, B23 is highly phosphorylated by Cdc2 kinase during mitosis, which is important for regulating B23 function and interactions during cell division. Anti-B23 antibody (3F291) is an invaluable tool for researchers studying nucleolar dynamics, ribosome assembly, and the intricate processes of cellular proliferation, providing insights into the fundamental mechanisms that govern cell growth and development.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

B23/Nucleophosmin Antibody (3F291)

sc-70392
200 µg/ml
$316.00

B23/Nucleophosmin Antibody (3F291): m-IgG Fc BP-HRP Bundle

sc-539270
200 µg Ab; 10 µg BP
$354.00

B23/Nucleophosmin Antibody (3F291): m-IgG1 BP-HRP Bundle

sc-541368
200 µg Ab; 20 µg BP
$354.00

What is the recommended starting dilution for Western blot for sc-70392: B23 (3F291) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from Does not work for IF in mouse neruonsTested free sample on ICC with dilution of 1:25 and 1:100 in primary neurons from mouse cortex. Does not work at all for ICC
Date published: 2018-12-05
Rated 4 out of 5 by from Positive result in WB in human B23 transfectedPositive result in WB in human B23 transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-14
Rated 4 out of 5 by from Published data for IP in HEK cellsPublished data for IP in HEK cells. -SCBT Publication Review
Date published: 2015-01-11
Rated 4 out of 5 by from Band of interest observed in WB in humanBand of interest observed in WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date published: 2013-01-12
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B23/Nucleophosmin Antibody (3F291) is rated 3.4 out of 5 by 5.
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