ATRX Antibody (D-5): sc-55584

ATRX Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects ATRX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-ATRX antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATRX plays a crucial role in chromatin remodeling and gene regulation, as a member of the SNF2 family of helicase/ATPases that facilitate dynamic restructuring of nucleosomes in an ATP-dependent manner, essential for proper transcription and replication processes. ATRX is characterized by a PHD zinc finger motif, important for interaction with specific chromatin components. ATRX localization is tightly regulated throughout the cell cycle; during interphase, ATRX associates with the nuclear matrix, while redistributing to condensed chromatin during mitosis. At M phase onset, ATRX undergoes rapid phosphorylation, relocating to short arms of human acrocentric chromosomes, forming a complex with heterochromatin protein 1 α, playing a vital role in chromosomal segregation. Mutations in ATRX are linked to severe X-linked syndromal mental retardation associated with α thalassemia, known as ATR-X syndrome, highlighting ATRX significance in cellular function and human health.

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ATRX Antibody (D-5) References:

1. Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.  |  McDowell, TL., et al. 1999. Proc Natl Acad Sci U S A. 96: 13983-8. PMID: 10570185
2. New mutations in XNP/ATR-X gene: a further contribution to genotype/phenotype relationship in ATR/X syndrome. Mutations in brief no. 176. Online.  |  Fichera, M., et al. 1998. Hum Mutat. 12: 214. PMID: 10660327
3. Cell cycle-dependent phosphorylation of the ATRX protein correlates with changes in nuclear matrix and chromatin association.  |  Bérubé, NG., et al. 2000. Hum Mol Genet. 9: 539-47. PMID: 10699177
4. ATR-X mutations cause impaired nuclear location and altered DNA binding properties of the XNP/ATR-X protein.  |  Cardoso, C., et al. 2000. J Med Genet. 37: 746-51. PMID: 11015451
5. Expression of DjXnp, a novel member of the SNF2-like ATP-dependent chromatin remodelling genes, in intact and regenerating planarians.  |  Rossi, L., et al. 2003. Int J Dev Biol. 47: 293-8. PMID: 12755334
6. The ATRX syndrome protein forms a chromatin-remodeling complex with Daxx and localizes in promyelocytic leukemia nuclear bodies.  |  Xue, Y., et al. 2003. Proc Natl Acad Sci U S A. 100: 10635-40. PMID: 12953102
7. ATRX/EZH2 complex epigenetically regulates FADD/PARP1 axis, contributing to TMZ resistance in glioma.  |  Han, B., et al. 2020. Theranostics. 10: 3351-3365. PMID: 32194873
8. DAXX-ATRX regulation of p53 chromatin binding and DNA damage response.  |  Gulve, N., et al. 2022. Nat Commun. 13: 5033. PMID: 36028493
9. Glioneuronal tumor with ATRX alteration, kinase fusion and anaplastic features (GTAKA): a molecularly distinct brain tumor type with recurrent NTRK gene fusions.  |  Bogumil, H., et al. 2023. Acta Neuropathol. 145: 667-680. PMID: 36933012
10. Interplay between ATRX and IDH1 mutations governs innate immune responses in diffuse gliomas.  |  Hariharan, S., et al. 2024. Nat Commun. 15: 730. PMID: 38272925
11. ATRX encodes a novel member of the SNF2 family of proteins: mutations point to a common mechanism underlying the ATR-X syndrome.  |  Picketts, DJ., et al. 1996. Hum Mol Genet. 5: 1899-907. PMID: 8968741
12. Determination of the genomic structure of the XNP/ATRX gene encoding a potential zinc finger helicase.  |  Villard, L., et al. 1997. Genomics. 43: 149-55. PMID: 9244431

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATRX Antibody (D-5) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-ATRX Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATRX Antibody (D-5) Specifications

Alternate Product Name: ATRX

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2193-2492

Epitope Target: ATRX

Epitope Species: human

Product Reactivity/Detection: ATRX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for ATRX Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin