ATP5B Antibody (A-8): sc-74549

ATP5B Antibody (A-8) is a mouse monoclonal IgG1 antibody that detects ATP5B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATP5B, also known as ATPMB or ATPSB, is a crucial 529 amino acid protein that plays a vital role in the mitochondrial membrane as a subunit of the F0 complex of mitochondrial ATP synthase. ATP5B is essential for ATP synthesis during oxidative phosphorylation, a process that generates the energy currency of the cell. The F0 complex, which includes ATP5B, forms a proton channel that is integral to the electrochemical gradient necessary for ATP production. ATP5B functions critically in energy metabolism and influences cellular proliferation and survival, particularly in high-energy-demand tissues such as the heart. ATP5B is encoded by a nuclear gene and assembles with other subunits encoded by both mitochondrial and nuclear genes, highlighting the intricate coordination between these two genetic systems. ATP5B mRNA expression varies significantly among different tissues, with elevated levels found in the heart, moderate levels in skeletal muscle, and minimal levels in the liver and kidney, indicating specialized roles in energy metabolism across various physiological contexts.

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ATP5B Antibody (A-8) References:

1. A monoclonal antibody against F1-F0 ATP synthase beta subunit.  |  Yuan, J., et al. 2012. Hybridoma (Larchmt). 31: 352-7. PMID: 23098302
2. Induction of ATP synthase β by H2O2 induces melanogenesis by activating PAH and cAMP/CREB/MITF signaling in melanoma cells.  |  Kim, HE. and Lee, SG. 2013. Int J Biochem Cell Biol. 45: 1217-22. PMID: 23523934
3. Possible Involvement of F1F0-ATP synthase and Intracellular ATP in Keratinocyte Differentiation in normal skin and skin lesions.  |  Xiaoyun, X., et al. 2017. Sci Rep. 7: 42672. PMID: 28209970
4. Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis.  |  Li, W., et al. 2017. Int J Oncol. 50: 1312-1320. PMID: 28259978
5. The role of formation of pyrrole-ATP synthase subunit beta adduct in pyrrolizidine alkaloid-induced hepatotoxicity.  |  Lu, Y., et al. 2018. Arch Toxicol. 92: 3403-3414. PMID: 30244272
6. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients.  |  Mota, MVB., et al. 2019. Cell Mol Neurobiol. 39: 149-160. PMID: 30539418
7. Profiling of rotavirus 3'UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication.  |  Ren, L., et al. 2019. J Biol Chem. 294: 5993-6006. PMID: 30770472
8. A novel RNA aptamer identifies plasma membrane ATP synthase beta subunit as an early marker and therapeutic target in aggressive cancer.  |  Speransky, S., et al. 2019. Breast Cancer Res Treat. 176: 271-289. PMID: 31006104
9. ATP synthase modulation leads to an increase of spare respiratory capacity in HPV associated cancers.  |  Kirschberg, M., et al. 2020. Sci Rep. 10: 17339. PMID: 33060693
10. Flagellar Hook Protein FlgE Induces Microvascular Hyperpermeability via Ectopic ATP Synthase β on Endothelial Surface.  |  Li, Y., et al. 2021. Front Cell Infect Microbiol. 11: 724912. PMID: 34796124

The preferred ATP5B antibody is ATP5B Antibody (E-1): sc-55597.