ATP-citrate synthase Antibody (5F8D11): sc-517267

ATP-citrate synthase Antibody (5F8D11) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATP-citrate synthase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-ATP-citrate synthase antibody (5F8D11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATP-citrate synthase, also known as ATP-citrate lyase or citrate cleavage enzyme, is a cytoplasmic homotetramer that belongs to the succinate/malate CoA ligase family, with its gene located on chromosome 17q12-q21. This enzyme plays a crucial role in cellular metabolism by catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate. Acetyl-CoA is a vital substrate for fatty acid and cholesterol biosynthesis, making ATP-citrate synthase essential for energy production and lipid metabolism. Additionally, ATP-citrate synthase is significant in the biosynthesis of acetylcholine in nervous tissue, highlighting its importance in neurotransmission and overall brain function.

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ATP-citrate synthase Antibody (5F8D11) References:

1. Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins.  |  Sato, R., et al. 2000. J Biol Chem. 275: 12497-502. PMID: 10777536
2. Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter.  |  Moon, YA., et al. 2002. Exp Mol Med. 34: 60-8. PMID: 11989980
3. The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes.  |  Berwick, DC., et al. 2002. J Biol Chem. 277: 33895-900. PMID: 12107176
4. Reverse genetic characterization of cytosolic acetyl-CoA generation by ATP-citrate lyase in Arabidopsis.  |  Fatland, BL., et al. 2005. Plant Cell. 17: 182-203. PMID: 15608338
5. Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: a window on renal citrate metabolism.  |  Tosukhowong, P., et al. 2005. Int J Urol. 12: 140-4. PMID: 15733107
6. Hepatic proteomic analysis of selenoprotein F knockout mice by iTRAQ: An implication for the roles of selenoprotein F in metabolism and diseases.  |  Zheng, X., et al. 2020. J Proteomics. 215: 103653. PMID: 31958640
7. Disturbed mitochondrial acetylation in accordance with the availability of acetyl groups in hepatocellular carcinoma.  |  Hu, X., et al. 2021. Mitochondrion. 60: 150-159. PMID: 34375734
8. Role of sodium dependent SLC13 transporter inhibitors in various metabolic disorders.  |  Akhtar, MJ., et al. 2023. Mol Cell Biochem. 478: 1669-1687. PMID: 36495372
9. Impacts of 2-bromoethanesulfonic sodium on methanogenesis: Methanogen metabolism and community structure.  |  Qiu, S., et al. 2023. Water Res. 230: 119527. PMID: 36580800
10. Morusin, a novel inhibitor of ACLY, induces mitochondrial apoptosis in hepatocellular carcinoma cells through ROS-mediated mitophagy.  |  Li, D., et al. 2024. Biomed Pharmacother. 180: 117510. PMID: 39341077
11. Variant cDNA sequences of human ATP:citrate lyase: cloning, expression, and purification from baculovirus-infected insect cells.  |  Lord, KA., et al. 1997. Protein Expr Purif. 9: 133-41. PMID: 9116495
12. ATP-citrate lyase deficiency in the mouse.  |  Beigneux, AP., et al. 2004. J Biol Chem. 279: 9557-64. PMID: 14662765

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATP-citrate synthase Antibody (5F8D11) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-ATP-citrate synthase Antibody (5F8D11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATP-citrate synthase Antibody (5F8D11) Specifications

Alternate Product Name: ATP-citrate synthase

Product Clone Name: 5F8D11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 306-502

Epitope Target: ATP-citrate synthase

Epitope Species: human

Product Reactivity/Detection: ATP-citrate synthase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for ATP-citrate synthase Antibody (5F8D11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-ATP-citrate synthase antibody is p-ATP-citrate synthase Antibody (A-12): sc-374647.