Atlastin Antibody (B-12): sc-374175

Atlastin Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Atlastin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Atlastin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Atlastin proteins, also known as SPG3A or guanylate-binding protein 3, are integral membrane proteins localized to the Golgi apparatus and belong to the Dynamin superfamily, which is crucial for maintaining axonal integrity. Proper functioning of Atlastin plays a significant role in the dynamics of membrane fusion and fission, processes essential for neuronal health and communication. Disruptions in these processes can lead to hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by the degeneration of long corticospinal neurons, resulting in spasticity and weakness in the lower extremities. Notably, Atlastin interacts with Spastin, a microtubule-severing AAA ATPase, implicated in the regulation of membrane dynamics. This interaction between Spastin and Atlastin serves as a critical component in the biochemical pathways contributing to HSP development. Mutations in the Atlastin gene (SPG3A) are responsible for approximately 10% of all autosomal dominant cases of HSP, while mutations in the Spastin gene (SPG4) account for nearly 40%, highlighting these proteins′ importance in neurodegenerative diseases.

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Atlastin Antibody (B-12) References:

1. Cellular localization, oligomerization, and membrane association of the hereditary spastic paraplegia 3A (SPG3A) protein atlastin.  |  Zhu, PP., et al. 2003. J Biol Chem. 278: 49063-71. PMID: 14506257
2. Early onset autosomal dominant spastic paraplegia caused by novel mutations in SPG3A.  |  Abel, A., et al. 2004. Neurogenetics. 5: 239-43. PMID: 15517445
3. Beginning to understand hereditary spastic paraplegia atlastin.  |  Elliott, JL. 2004. Arch Neurol. 61: 1842-3. PMID: 15596602
4. Atlastin1 mutations are frequent in young-onset autosomal dominant spastic paraplegia.  |  Dürr, A., et al. 2004. Arch Neurol. 61: 1867-72. PMID: 15596607
5. The R495W mutation in SPG3A causes spastic paraplegia associated with axonal neuropathy.  |  Scarano, V., et al. 2005. J Neurol. 252: 901-3. PMID: 15742100
6. Mutation analysis of SPG4 and SPG3A genes and its implication in molecular diagnosis of Korean patients with hereditary spastic paraplegia.  |  Park, SY., et al. 2005. Arch Neurol. 62: 1118-21. PMID: 16009769
7. Spinal cord magnetic resonance imaging in autosomal dominant hereditary spastic paraplegia.  |  Hedera, P., et al. 2005. Neuroradiology. 47: 730-4. PMID: 16143870
8. SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development.  |  Zhu, PP., et al. 2006. Hum Mol Genet. 15: 1343-53. PMID: 16537571
9. Interaction of two hereditary spastic paraplegia gene products, spastin and atlastin, suggests a common pathway for axonal maintenance.  |  Evans, K., et al. 2006. Proc Natl Acad Sci U S A. 103: 10666-71. PMID: 16815977
10. Atlastin-1 modulates seizure activity and neuronal excitability.  |  Lu, X., et al. 2020. CNS Neurosci Ther. 26: 385-393. PMID: 31729196
11. The atlastin paralogs: The complexity in the tails.  |  Krishna, S. and Ford, MGJ. 2023. J Cell Biol. 222: PMID: 37327452
12. Atlastin-1 regulates endosomal tubulation and lysosomal proteolysis in human cortical neurons.  |  Zlamalova, E., et al. 2024. Neurobiol Dis. 199: 106556. PMID: 38851544

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Atlastin Antibody (B-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Atlastin Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Atlastin Antibody (B-12) Specifications

Alternate Product Name: ATL1

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 514-550

Epitope Target: Atlastin

Epitope Species: human

Product Reactivity/Detection: Atlastin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Atlastin Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin