APG5/ATG5 Antibody (E-9): sc-515347
- APG5/ATG5 Antibody (E-9) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 250-275 at the C-terminus of ATG5 of human origin
- recommended for detection of ATG5 long and short isoforms of human origin and ATG5 of mouse and rat origin by WB, IP, IF and ELISA
- See APG5/ATG5 (C-1): sc-133158 for APG5/ATG5 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for APG5/ATG5 Antibody (E-9) for WB applications. This reagent is now offered in a bundle with APG5/ATG5 Antibody (E-9) (see ordering information below).
QUICK LINKS
ATG5 Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody developed to detect ATG5, also known as autophagy-related 5 (APG5) or apoptosis-specific protein (ASP). Anti-ATG5 antibody (E-9) recognizes an epitope spanning amino acids 250-275 at the C-terminus of human ATG5, ensuring reliable detection in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATG5 is a crucial component of the autophagy machinery, a fundamental cellular process responsible for degradation and recycling of cytoplasmic components to maintain cellular homeostasis, especially under stress conditions like nutrient starvation. Located primarily in the cytoplasm, ATG5 translocates to the forming autophagosome membrane where ATG5 forms a complex with ATG12 and ATG16L1, facilitating elongation and closure of autophagosomes. This localization is essential for proper formation of autophagosomes, which sequester damaged organelles and proteins for degradation, thereby preventing accumulation of cellular debris and promoting cell survival. ATG5 plays a role in regulation of apoptosis, linking autophagy to programmed cell death pathways and influencing cell fate decisions. ATG5′s interaction with other autophagy-related proteins underscores ATG5′s importance in orchestrating complex mechanisms of cellular degradation and recycling. Anti-ATG5 antibody (E-9) enables researchers to effectively study spatial and temporal dynamics of ATG5 within cells, providing insights into regulation of autophagy and implications in various diseases such as cancer, neurodegeneration, and infections. ATG5 (E-9) antibody serves as an indispensable tool for experiments investigating localization and function of ATG5, facilitating advancements in understanding cellular homeostasis and identifying potential therapeutic targets for a range of pathological conditions.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APG5/ATG5 Antibody (E-9) | sc-515347 | 200 µg/ml | $316.00 | |||
APG5/ATG5 Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-541050 | 200 µg Ab; 10 µg BP | $354.00 | |||
APG5/ATG5 (E-9) Neutralizing Peptide | sc-515347 P | 100 µg/0.5 ml | $68.00 |