ATF4 Antibody (B-3): sc-390063

ATF-4 Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody, specifically developed to recognize Activating Transcription Factor 4 (ATF-4), also known as CREB-2, across mouse, rat, and human species. ATF-4 monoclonal antibody (B-3) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) applications, making ATF-4 (B-3) antibody a versatile tool for various research settings. ATF-4 is a critical transcription factor involved in regulating gene expression in response to cellular stress, including the unfolded protein response, amino acid deprivation, and oxidative stress. By binding to specific DNA sequences, ATF-4 modulates the expression of genes that control essential cellular processes such as metabolism, differentiation, and apoptosis. The functionality of ATF-4 is pivotal for maintaining cellular homeostasis and enabling cells to adapt to adverse conditions, thereby playing a significant role in development, immune response, and disease progression. Structurally, ATF-4 contains a basic leucine zipper (bZIP) domain that facilitates dimerization and DNA binding, essential for its role in transcriptional regulation. Additionally, ATF-4 interacts with other members of the ATF/CREB family, forming homo- or heterodimers that enhance its regulatory capabilities. Dysregulation of ATF-4 has been implicated in various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders, highlighting its importance as a target for therapeutic intervention. ATF-4 (B-3) monoclonal antibody supports advanced research into the molecular mechanisms governing cellular stress responses and their implications in health and disease.

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ATF4 Antibody (B-3) References:

1. Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity.  |  Hai, T. and Curran, T. 1991. Proc Natl Acad Sci U S A. 88: 3720-4. PMID: 1827203
2. A cDNA for a human cyclic AMP response element-binding protein which is distinct from CREB and expressed preferentially in brain.  |  Kara, CJ., et al. 1990. Mol Cell Biol. 10: 1347-57. PMID: 2320002
3. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
4. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.  |  Maekawa, T., et al. 1989. EMBO J. 8: 2023-8. PMID: 2529117
5. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
6. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
7. Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.  |  Hoeffler, JP., et al. 1988. Science. 242: 1430-3. PMID: 2974179
8. ATF-4 and hydrogen sulfide signalling mediate longevity in response to inhibition of translation or mTORC1.  |  Statzer, C., et al. 2022. Nat Commun. 13: 967. PMID: 35181679
9. PERK is a critical metabolic hub for immunosuppressive function in macrophages.  |  Raines, LN., et al. 2022. Nat Immunol. 23: 431-445. PMID: 35228694
10. β-(4-fluorobenzyl) Arteannuin B induced interaction of ATF-4 and C/EBPβ mediates the transition of breast cancer cells from autophagy to senescence.  |  Mir, KB., et al. 2022. Front Oncol. 12: 1013500. PMID: 36465376
11. CREB-1 and CREB-2 immunoreactivity in the rat brain.  |  Ferrer, I., et al. 1996. Brain Res. 712: 159-64. PMID: 8705300
12. CREB-2, a cellular CRE-dependent transcription repressor, functions in association with Tax as an activator of the human T-cell leukemia virus type 1 promoter.  |  Gachon, F., et al. 1998. J Virol. 72: 8332-7. PMID: 9733879

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF4 Antibody (B-3) has 118 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF4 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF4 Antibody (B-3) Specifications

Alternate Product Name: ATF-4

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-290

Epitope Target: ATF-4

Epitope Species: human

Product Reactivity/Detection: ATF-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 118 citations in various scientific publications

Available Conjugates/Forms for ATF4 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent