ATF-2 Antibody (F2BR-1): sc-242

ATF-2 Antibody (F2BR-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATF-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). ATF-2 (F2BR-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATF-2 protein plays a crucial role in regulating gene expression, particularly in response to stress signals, by functioning as a transcription factor that binds to specific DNA sequences known as cAMP response elements (CREs). This binding is essential for transcriptional activation of genes involved in cellular stress responses, cell proliferation, and differentiation. ATF-2 can form homodimers or heterodimers with other proteins, such as c-Jun, which enhances transcriptional regulation ability. ATF-2 undergoes post-translational modifications, including phosphorylation at specific threonine residues, which are critical for activation and stability. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is particularly important for transcriptional activity, while Myc-induced phosphorylation also contributes to stability. ATF-2 exhibits histone acetyltransferase activity, specifically acetylating histones H2B and H4, thereby influencing chromatin structure and gene accessibility. This multifaceted role of ATF-2 underscores importance in cellular responses to environmental cues and potential implications in various diseases, including cancer and neurodegenerative disorders.

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ATF-2 Antibody (F2BR-1) References:

1. ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation.  |  Kawasaki, H., et al. 2000. Nature. 405: 195-200. PMID: 10821277
2. Crosstalk between Myc and activating transcription factor 2 (ATF2): Myc prolongs the half-life and induces phosphorylation of ATF2.  |  Miethe, J., et al. 2001. Oncogene. 20: 8116-24. PMID: 11781825
3. The role of ATF-2 in oncogenesis.  |  Vlahopoulos, SA., et al. 2008. Bioessays. 30: 314-27. PMID: 18348191
4. Assignment of the gene for cyclic AMP-response element binding protein 2 (CREB2) to human chromosome 2q24.1-q32.  |  Diep, A., et al. 1991. Genomics. 11: 1161-3. PMID: 1838349
5. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
6. ATF-2 immunoreactivity in post-mitotic and terminally differentiated human odontoblasts.  |  Keklikoglu, N. and Akinci, S. 2015. Med Mol Morphol. 48: 164-8. PMID: 25417007
7. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
8. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
9. ERK1/ATF-2 signaling axis contributes to interleukin-1β-induced MMP-3 expression in dermal fibroblasts.  |  Kitanaka, N., et al. 2019. PLoS One. 14: e0222869. PMID: 31536594
10. Overexpression of Activating Transcription Factor-2 (ATF-2) Activates Wnt/Ca2+ Signaling Pathways and Promotes Proliferation and Invasion in Non-Small-Cell Lung Cancer.  |  Zhang, L., et al. 2022. Dis Markers. 2022: 5772089. PMID: 35692887
11. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents.  |  van Dam, H., et al. 1995. EMBO J. 14: 1798-811. PMID: 7737130
12. Heterodimer formation of cJun and ATF-2 is responsible for induction of c-jun by the 243 amino acid adenovirus E1A protein.  |  van Dam, H., et al. 1993. EMBO J. 12: 479-87. PMID: 8382609

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF-2 Antibody (F2BR-1) has 86 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF-2 Antibody (F2BR-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF-2 Antibody (F2BR-1) Specifications

Alternate Product Name: ATF2

Product Clone Name: F2BR-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 350-505

Epitope Target: ATF-2

Epitope Species: human

Product Reactivity/Detection: ATF-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 86 citations in various scientific publications

Available Conjugates/Forms for ATF-2 Antibody (F2BR-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-ATF-2 antibody is p-ATF-2 Antibody (F-1): sc-8398.