Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATE1 CRISPR/Cas9 KO Plasmid (h): sc-405539
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATE1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ATE1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATE1 Antibody (G-6): sc-398805
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATE1 CRISPR/Cas9 KO Plasmid (h) | sc-405539 | 20 µg | $397.00 |
Overview
ATE1 encodes arginyltransferase 1, a key enzyme of the N-end rule pathway that post-translationally adds arginine to protein N-termini, creating degrons that direct substrates to ubiquitin-dependent proteasomal turnover. Through regulation of protein stability, ATE1 influences proteostasis, cytoskeletal organization, and stress-responsive signaling, and has been linked to control of cell migration and adhesion via modulation of actin-associated proteins. ATE1-dependent arginylation also contributes to mitochondrial and oxidative stress adaptation by shaping the abundance of select regulators. Dysregulation of N-end rule components, including ATE1, is associated with altered cellular homeostasis relevant to cancer biology, neurodegeneration, and developmental phenotypes in experimental systems.
ATE1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ATE1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ATE1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ATE1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ATE1 protein expression.
This CRISPR knockout system enables efficient generation of ATE1-deficient cell models for investigation of ATE1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ATE1 exon(s) critical for ATE1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ATE1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ATE1 CRISPR/Cas9 KO Plasmid (h) and ATE1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ATE1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ATE1 HDR Plasmid (h) and ATE1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ATE1 homology arms to support homology-directed repair at defined ATE1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.