Ataxin-1 Antibody (E-4): sc-514953

Ataxin-1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ataxin-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ataxin-1 antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ataxin-1, also known as spinocerebellar ataxia type 1 protein (SCA-1), plays a crucial role in regulating gene expression and cellular signaling pathways, particularly in the central nervous system. Proper Ataxin-1 function is vital for maintaining neuronal health, and mutations in Ataxin-1 are linked to the autosomal dominant neurodegenerative disorder spinocerebellar ataxia type 1 (SCA-1), which leads to progressive neuronal loss, muscle wasting, and ataxia. Ataxin-1 is predominantly localized in the cytoplasm and nucleus, where Ataxin-1 interacts with key proteins such as the Purkinje-enriched leucine-rich acidic nuclear protein (LANP) and the nuclear matrix-associated protein promyelocytic leukemia protein (PML). In SCA-1, Ataxin-1 undergoes a mutation resulting in a polyglutamine expansion, leading to toxic nuclear aggregate formation that disrupts normal cellular functions and exacerbates disease pathology. Anti-Ataxin-1 antibody (E-4)′s ability to effectively detect Ataxin-1 across multiple species and applications makes this product an invaluable tool for researchers studying mechanisms underlying SCA-1 and related neurodegenerative conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Ataxin-1 Antibody (E-4) References:

1. Ataxin-1 occupies the promoter region of E-cadherin in vivo and activates CtBP2-repressed promoter.  |  Lee, S., et al. 2011. Biochim Biophys Acta. 1813: 713-22. PMID: 21315774
2. Ataxin-1 and Brother of ataxin-1 are components of the Notch signalling pathway.  |  Tong, X., et al. 2011. EMBO Rep. 12: 428-35. PMID: 21475249
3. Mutant Ataxin-1 Inhibits Neural Progenitor Cell Proliferation in SCA1.  |  Cvetanovic, M., et al. 2017. Cerebellum. 16: 340-347. PMID: 27306906
4. Ataxin-1 is involved in tumorigenesis of cervical cancer cells via the EGFR-RAS-MAPK signaling pathway.  |  Kang, AR., et al. 2017. Oncotarget. 8: 94606-94618. PMID: 29212253
5. Dual-specificity phosphatase 18 modulates the SUMOylation and aggregation of Ataxin-1.  |  Ryu, J. and Lee, DH. 2018. Biochem Biophys Res Commun. 502: 389-396. PMID: 29852174
6. A Structural Study of the Cytoplasmic Chaperone Effect of 14-3-3 Proteins on Ataxin-1.  |  Leysen, S., et al. 2021. J Mol Biol. 433: 167174. PMID: 34302818
7. SCA1 transgenic mice: a model for neurodegeneration caused by an expanded CAG trinucleotide repeat.  |  Burright, EN., et al. 1995. Cell. 82: 937-48. PMID: 7553854
8. Identification and characterization of the gene causing type 1 spinocerebellar ataxia.  |  Banfi, S., et al. 1994. Nat Genet. 7: 513-20. PMID: 7951322
9. Identification of a self-association region within the SCA1 gene product, ataxin-1.  |  Burright, EN., et al. 1997. Hum Mol Genet. 6: 513-8. PMID: 9097953
10. Ataxin-1 with an expanded glutamine tract alters nuclear matrix-associated structures.  |  Skinner, PJ., et al. 1997. Nature. 389: 971-4. PMID: 9353120
11. The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1.  |  Matilla, A., et al. 1997. Nature. 389: 974-8. PMID: 9353121
12. Ataxin-1 nuclear localization and aggregation: role in polyglutamine-induced disease in SCA1 transgenic mice.  |  Klement, IA., et al. 1998. Cell. 95: 41-53. PMID: 9778246

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ataxin-1 Antibody (E-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ataxin-1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ataxin-1 Antibody (E-4) Specifications

Alternate Product Name: Ataxin-1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 578-752

Epitope Target: Ataxin-1

Epitope Species: human

Product Reactivity/Detection: Ataxin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ataxin-1 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin