ASTL Antibody (D-8): sc-514054

ASTL Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects ASTL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASTL (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASTL protein, also known as astacin-like metalloendopeptidase or ovastacin, is a 435 amino acid protein that plays a crucial role in fertilization by regulating the zona pellucida, a glycoprotein layer surrounding the oocyte. This regulation prevents polyspermy, ensuring that only one sperm fertilizes the egg, which is essential for proper embryonic development. Highly expressed in unfertilized oocytes, ASTL levels drop significantly upon fertilization, indicating specific function during early stages of reproduction. Human ASTL protein shares 78% sequence identity with mouse counterpart and features an N-terminal signal peptide, a prodomain, a zinc-dependent metalloprotease domain, and a C-terminal extension that is likely heavily O-glycosylated. ASTL gene is located on chromosome 2q11.2, and two isoforms of ASTL2 arise from alternative splicing events. ASTL′s catalytic activity is inhibited by EDTA and the broad-spectrum metalloproteinase inhibitor batimastat (BB-94), highlighting potential as a target for therapeutic interventions in reproductive health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ASTL Antibody (D-8) References:

1. Identification and characterization of human and mouse ovastacin: a novel metalloproteinase similar to hatching enzymes from arthropods, birds, amphibians, and fish.  |  Quesada, V., et al. 2004. J Biol Chem. 279: 26627-34. PMID: 15087446
2. Meprins, membrane-bound and secreted astacin metalloproteinases.  |  Sterchi, EE., et al. 2008. Mol Aspects Med. 29: 309-28. PMID: 18783725
3. Special issue: metzincin metalloproteinases.  |  Sterchi, EE. 2008. Mol Aspects Med. 29: 255-7. PMID: 18796311
4. News from an ancient world: two novel astacin metalloproteases from the horseshoe crab.  |  Becker-Pauly, C., et al. 2009. J Mol Biol. 385: 236-48. PMID: 18996129
5. [The astacin family of metalloproteinases].  |  Semenova, SA. and Rudenskaia, GN. 2008. Biomed Khim. 54: 531-54. PMID: 19105396
6. The astacin family of metalloendopeptidases.  |  Bond, JS. and Beynon, RJ. 1995. Protein Sci. 4: 1247-61. PMID: 7670368
7. Implications of the three-dimensional structure of astacin for the structure and function of the astacin family of zinc-endopeptidases.  |  Stöcker, W., et al. 1993. Eur J Biochem. 214: 215-31. PMID: 8508794

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASTL Antibody (D-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ASTL Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASTL Antibody (D-8) Specifications

Alternate Product Name: ASTL

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2-207

Epitope Target: ASTL

Epitope Species: mouse

Product Reactivity/Detection: ASTL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ASTL Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin