Asparagine synthetase Antibody (G-10): sc-365809

Asparagine synthetase Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Asparagine synthetase (ASNS) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ASNS antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASNS, also known as cell cycle control protein TS11, plays a crucial role in amino acid biosynthesis, particularly in the synthesis of L-asparagine from L-aspartate via the L-glutamine pathway. ASNS is essential for protein synthesis and cell proliferation. ASNS features one asparagine synthetase domain and one type-2 glutamine amidotransferase domain, which are critical for enzymatic activity. ASNS gene regulation occurs during the cell cycle, making ASNS necessary for G1 phase progression, thereby linking ASNS function to cell growth and division.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Asparagine synthetase Antibody (G-10) References:

1. Upregulation of asparagine synthetase fails to avert cell cycle arrest induced by L-asparaginase in TEL/AML1-positive leukaemic cells.  |  Krejci, O., et al. 2004. Leukemia. 18: 434-41. PMID: 14724653
2. Amino acid deprivation induces the transcription rate of the human asparagine synthetase gene through a timed program of expression and promoter binding of nutrient-responsive basic region/leucine zipper transcription factors as well as localized histone acetylation.  |  Chen, H., et al. 2004. J Biol Chem. 279: 50829-39. PMID: 15385533
3. A genome-wide view of the in vitro response to l-asparaginase in acute lymphoblastic leukemia.  |  Fine, BM., et al. 2005. Cancer Res. 65: 291-9. PMID: 15665306
4. Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase.  |  Greco, A., et al. 1989. Mol Cell Biol. 9: 2350-9. PMID: 2569668
5. The N-terminal cysteine of human asparagine synthetase is essential for glutamine-dependent activity.  |  Van Heeke, G. and Schuster, SM. 1989. J Biol Chem. 264: 19475-7. PMID: 2573597
6. Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells.  |  Andrulis, IL., et al. 1987. Mol Cell Biol. 7: 2435-43. PMID: 2886907
7. Asparagine synthetase: Function, structure, and role in disease.  |  Lomelino, CL., et al. 2017. J Biol Chem. 292: 19952-19958. PMID: 29084849
8. Asparagine synthetase regulates lung-cancer metastasis by stabilizing the β-catenin complex and modulating mitochondrial response.  |  Cai, DJ., et al. 2022. Cell Death Dis. 13: 566. PMID: 35739087
9. Transcriptional repressor RST1 controls salt tolerance and grain yield in rice by regulating gene expression of asparagine synthetase.  |  Deng, P., et al. 2022. Proc Natl Acad Sci U S A. 119: e2210338119. PMID: 36472959
10. Primary cilia sense glutamine availability and respond via asparagine synthetase.  |  Steidl, ME., et al. 2023. Nat Metab. 5: 385-397. PMID: 36879119
11. Asparagine Synthetase Marks a Distinct Dependency Threshold for Cardiomyocyte Dedifferentiation.  |  Zhu, Y., et al. 2024. Circulation. 149: 1833-1851. PMID: 38586957
12. Inhibition of asparagine synthetase effectively retards polycystic kidney disease progression.  |  Clerici, S., et al. 2024. EMBO Mol Med. 16: 1379-1403. PMID: 38684863

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Asparagine synthetase Antibody (G-10) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-Asparagine synthetase Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Asparagine synthetase Antibody (G-10) Specifications

Alternate Product Name: Asparagine synthetase

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 171-200

Epitope Target: Asparagine synthetase

Epitope Species: human

Product Reactivity/Detection: Asparagine synthetase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for Asparagine synthetase Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin